脐带间充质干细胞的生物学特性及旁分泌作用的研究进展

doi:10.19586/j.2095-2341.2022.0010

生物技术进展 ›› 2022, Vol. 12 ›› Issue (4): 559-567.DOI: 10.19586/j.2095-2341.2022.0010

脐带间充质干细胞的生物学特性及旁分泌作用的研究进展

陈小巧(), 牧仁(), 李玉玲

内蒙古师范大学生命科学与技术学院，呼和浩特 010022

收稿日期:2022-01-27 接受日期:2022-04-20 出版日期:2022-07-25 发布日期:2022-08-10
通讯作者: 牧仁
作者简介:陈小巧 E-mail：cxq1019@qq.com；
基金资助:
国家自然科学基金项目(31260265);内蒙古自治区自然科学基金项目(2019MS08166)

Research Progress on the Biological Characteristics and Paracrine Effects of Umbilical Cord Mesenchymal Stem Cells

Xiaoqiao CHEN(), Ren MU(), Yuling LI

College of Life Science and Technology，Inner Mongolia Normal University，Hohhot 010022，China

Received:2022-01-27 Accepted:2022-04-20 Online:2022-07-25 Published:2022-08-10
Contact: Ren MU

摘要/Abstract

摘要：

脐带是由胚胎外中胚层和/或胚胎中胚层发育而来的组织，脐带间充质干细胞是具有自我更新、多向分化以及高度增殖潜能的多功能干细胞。研究证明，脐带间充质干细胞具有以下功能：参与炎症反应，抑制炎症因子分泌并促进免疫调节；参与受损伤组织的治疗与修复使其再生并改善特定疾病症状；抑制肿瘤增殖和迁移以及促进其凋亡等。然而目前尚未明确以上功能是间充质干细胞本身发挥作用，还是其分泌的相关因子对机体修复产生作用。主要对脐带间充质干细胞的定义、来源、生物学特性、分泌功能等方面的研究进展进行了综述，旨在更好地利用间充质干细胞修复组织，以期为脐带间充质干细胞的后续研究提供参考依据。

关键词: 脐带间充质干细胞, 生物学特性, 免疫调节, 旁分泌作用, 抗肿瘤

Abstract:

The umbilical cord is a tissue developed from the embryonic outer mesoderm and/or embryonic mesoderm. Umbilical cord mesenchymal stem cells are multifunctional stem cells with self-renewal， multi-directional differentiation and high proliferation potential. Studies have shown that umbilical cord mesenchymal stem cells have following functions： participating in inflammatory response， inhibiting the secretion of inflammatory factors and promoting immune regulation； participating in the treatment and repair of injured tissue to regenerate and improving the symptoms of specific diseases； inhibiting tumor proliferation and migration and promoting its apoptosis. However， it is not clear whether the mesenchymal stem cells themselves or the related factors secreted by mesenchymal stem cells were responsible for body repair. This article mainly reviewed the progress in the definition， source， biological characteristics and secretory function of umbilical cord mesenchymal stem cells， which aimed to make use of the ability of mesenchymal stem cells to repair tissue， and provide references for the follow-up research of umbilical cord mesenchymal stem cells.

Key words: umbilical cord mesenchymal stem cells, biological characteristics, immunomodulatory, paracrine effects, anti-tumor

中图分类号:

R329.2

陈小巧, 牧仁, 李玉玲. 脐带间充质干细胞的生物学特性及旁分泌作用的研究进展[J]. 生物技术进展, 2022, 12(4): 559-567.

Xiaoqiao CHEN, Ren MU, Yuling LI. Research Progress on the Biological Characteristics and Paracrine Effects of Umbilical Cord Mesenchymal Stem Cells[J]. Current Biotechnology, 2022, 12(4): 559-567.

图/表 4

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来源	表面标志物
来源	阳性	阴性
骨髓^[19-20]	CD29、CD44、CD71、CD73、CD90、CD105（SH2）、CD106、CD120a、CD124、CD166（SH3）	CD14、CD34、CD45、HLA-DR
脐带血^[21]	CD29、CD44、CD73、CD105、CD106、HLAⅠ	CD14、CD34、CD45、CD133、HLAⅡ
华通氏胶^{[13-14, 16]}	CD13、CD29、CD44、CD73、CD90、CD105、CD117（C-kit）、HLAⅠ、OCT4、Sox2、Nanog和Nucleostemin	CD31、CD34、CD45、HLAⅡ、CD14
脐血管^{[15, 18]}	CD13、CD29、CD73、CD90、CD105、OCT-4、SSEA-4、Nanog、ZFX、Bmi-1、Nucleostemin	CD34、CD45、HLA-DR、SSEA-3、TRA-1-60、TRA-1-81
脐带内膜^[17]	CD44、CD73、CD105、CD106、CD166、STRO-1、SSEA-4、HLA-ABC	CD19、CD34、CD45、CD117、CD133、HLA-DR
脐带血管周围^[22]	CD44、CD73（SH3）、CD90（Thy-1）、CD105（SH2）、CD117（C-kit）、MHC I	CD34、CD45、CD235a、CD106、CD123、SSEA-4、HLA-DR、HLA-DP、HLA-DQ（MHCⅡ）、HLA-G、OCT-4

来源	表面标志物
来源	阳性	阴性
骨髓^[19-20]	CD29、CD44、CD71、CD73、CD90、CD105（SH2）、CD106、CD120a、CD124、CD166（SH3）	CD14、CD34、CD45、HLA-DR
脐带血^[21]	CD29、CD44、CD73、CD105、CD106、HLAⅠ	CD14、CD34、CD45、CD133、HLAⅡ
华通氏胶^{[13-14, 16]}	CD13、CD29、CD44、CD73、CD90、CD105、CD117（C-kit）、HLAⅠ、OCT4、Sox2、Nanog和Nucleostemin	CD31、CD34、CD45、HLAⅡ、CD14
脐血管^{[15, 18]}	CD13、CD29、CD73、CD90、CD105、OCT-4、SSEA-4、Nanog、ZFX、Bmi-1、Nucleostemin	CD34、CD45、HLA-DR、SSEA-3、TRA-1-60、TRA-1-81
脐带内膜^[17]	CD44、CD73、CD105、CD106、CD166、STRO-1、SSEA-4、HLA-ABC	CD19、CD34、CD45、CD117、CD133、HLA-DR
脐带血管周围^[22]	CD44、CD73（SH3）、CD90（Thy-1）、CD105（SH2）、CD117（C-kit）、MHC I	CD34、CD45、CD235a、CD106、CD123、SSEA-4、HLA-DR、HLA-DP、HLA-DQ（MHCⅡ）、HLA-G、OCT-4

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脐带间充质干细胞的生物学特性及旁分泌作用的研究进展

Research Progress on the Biological Characteristics and Paracrine Effects of Umbilical Cord Mesenchymal Stem Cells

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