固体发酵裂褶多糖免疫调节及抗肿瘤活性研究

doi:10.3969/j.issn.2095-2341.2013.06.09

生物技术进展 ›› 2013, Vol. 3 ›› Issue (6): 421-426.DOI: 10.3969/j.issn.2095-2341.2013.06.09

固体发酵裂褶多糖免疫调节及抗肿瘤活性研究

钟葵，佟立涛，刘丽娅，周闲容，钟昕，周素梅*

中国农业科学院农产品加工研究所，北京 100193

收稿日期:2013-10-08 出版日期:2013-11-25 发布日期:2013-11-12
通讯作者: 周素梅，研究员，主要从事粮油精深加工和生物活性多糖研究。E-mail：zhousumei1001@163.com
作者简介:钟葵，副教授，研究方向为生物活性多糖。
基金资助:
国家自然科学基金项目（31201312）资助。

Immunity-modulatory and Antitumor Activities of Schizophyllan Produced with Solid-state Fermentation

ZHONG Kui, TONG Li-tao, LIU Li-ya, ZHOU Xian-rong, ZHONG Xin, ZHOU Su -mei

Institute of Agro-products Processing Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received:2013-10-08 Online:2013-11-25 Published:2013-11-12

摘要/Abstract

摘要： 以麦麸和玉米芯为原料固体发酵生产裂褶多糖（schizophyllan，SPG），采用辐照技术降解处理SPG（irradiated schizophyllan，ISPG），研究辐照前后裂褶多糖分子量、体内免疫调节及抗肿瘤活性变化。结果显示，辐照能有效降低天然SPG 分子量，分子量从106~107 Da（SPG）降低到104~105 Da（ISPG）。SPG和ISPG均具有良好免疫调节和抗肿瘤活性，SPG和ISPG中剂量（100 mg/kg体重·d）和低剂量（ 50 mg/kg体重·d）组均对S180荷瘤小鼠羊红细胞诱导迟发型变态反应（DTH）和脾细胞介导羊红细胞定量溶血值（QHS）具有显著增强功效，ISGP中剂量组（100 mg/kg体重·d）能显著增强小鼠巨噬细胞吞噬鸡红细胞效果，提升小鼠非特异性免疫功能；SPG和ISPG不同剂量组抑瘤率均在45%以上，ISPG中剂量组（100 mg/kg体重 ·d）抑瘤率最高（74.80%±6.83%）。总体看来，辐照处理后的ISPG比天然SPG具有更好的免疫调节及抗肿瘤活性。

关键词: 裂褶多糖, 固体发酵, 辐照, 免疫调节活性, 抗肿瘤活性

Abstract: Schizophyllan (SPG) was produced by the fungus Schizophyllum commune under solid-state fermentation conditions in this study. Irradiation technology was used to treat SPG and molecular weight and biological effects of SPG and irradiated schizophyllan (ISPG) on the immunity-modulatory and antitumor activities in S180 tumor mice models in vivo were investigated. Irradiation technology decreased significantly the molecular weight of SPG (P< 0.05), and the range of molecular weight of SPG and ISPG were 106~107 Da and 104~105 Da, respectively. Both SPG and ISPG with medium dose (100 mg/kg) and low dose (50 mg/kg) had potent immune-modulatory effects in S180 tumor mice model and exhibited significant effect on delayed-type hypersensitivity (DTH) response and quantitative hemolysis spectrophotometry (QHS) test (P<0.05). Besides, ISPG with low dose (50 mg/kg) elevated significantly the phagocytosis of macrophage (P<0.05). The results still showed that SPG and ISPG had great antitumor effects, and ISPG at medium dose (100 mg/kg) had maximum inhibition rate (74.80%±6.83%). Therefore, ISPG had better immunity- modulatory and antitumor activities than natural SPG.

Key words: schizophyllan, solid-state fermentation, irradiation, immunity- modulatory activity, antitumor activity

钟葵，佟立涛，刘丽娅，周闲容，钟昕，周素梅. 固体发酵裂褶多糖免疫调节及抗肿瘤活性研究[J]. 生物技术进展, 2013, 3(6): 421-426.

ZHONG Kui, TONG Li-tao, LIU Li-ya, ZHOU Xian-rong, ZHONG Xin, ZHOU Su. Immunity-modulatory and Antitumor Activities of Schizophyllan Produced with Solid-state Fermentation[J]. Curr. Biotech., 2013, 3(6): 421-426.

固体发酵裂褶多糖免疫调节及抗肿瘤活性研究

Immunity-modulatory and Antitumor Activities of Schizophyllan Produced with Solid-state Fermentation

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