关键词: 壳聚糖薄膜, 间充质干细胞, 球体培养, 抗炎相关基因, 骨关节炎

Abstract: To compare the expression of anti-inflammatory genes in mesenchymal stem cells (MSCs) between chitosan membrane culture and conventional plate culture, MSCs was isolated from umbilical cord and cultured with chitosan membrane and normal plate culture respectively. The expression of LIF, IL6ST, IL-10, TSG6, COX-2, STC-1, IL-1RN, IL-4, IL-13 mRNA and their proteins were detected by Real-time PCR, Western blot and immunofluorescence. Results showed that,the MSCs morphology of chitosan film was spheroid, which could aggregate into multicellular spheroids. The expression levels of anti-inflammatory related genes LIF, IL6ST, IL-10, TSG6, COX-2, STC-1, IL-1RN, IL-4, IL-13 mRNA and their proteins in chitosan film-cultured MSCs were significantly higher than those in the conventional culture group, and the difference was statistically significant (P<0.01). Compared with conventional MSCs, chitosan membrane cultured MSCs could form spheroids, and the expression of anti-inflammatory genes was significantly increased.

Key words: chitosan membrane, mesenchymal stem cells, spheroid culture, anti-inflammatory related genes, osteoarthritis