关键词: 壳聚糖薄膜, 球体培养, 间充质干细胞, 迁徙趋化

Abstract: To investigate the effects of chitosan-mediated spheroid formation on the migration and chemotaxis of mesenchymal stem cells (MSCs). The original MSCs were extracted from umbilical cord tissue, and were cultured on chitosan(CS) membranes, compared with these on trational adherent substrate. MSCs of two groups were collected after 72 hours. Migration rate and chemotactic effect of MSCs were measured by wound healing assay and tranthwell migration assay, observed and screened the cell morphology. The expression levels of genes related to MSCs migration and chemotaxis in two cultures were further detected by real-time PCR（RT-PCR). The results showed that, compared with the MSCs derived on adherent substrate, there was a significant statistical difference (P<0.01) that MSC spheroids derived on CS have more active migration and chemotaxis in vitro. And the expression level of migration genes, such as CXCR4, CXCR7, MCP-1, MMP-1, MMP-2, MMP-9 and TIMP-2, was significantly up-regulated in the MSC spheroids derived on CS (P<0.01). The experimental results showed that chitosan-mediated spheroid formation could enhanced the activities of migration and chemotaxis of MSCs.

Key words: chitosan membranes, spheroid formation, mesenchymal stem cells, migration and chemotaxis