关键词: RNA结合蛋白, RNA, RBP-RNA相互作用, 紫外交联免疫沉淀

Abstract: RNA binding proteins (RBPs) are critical regulators of transcriptional and posttranscriptional gene expression in eukaryotic organisms, and they are involved in many important cell biological processes including the coordination and stabilization of protein complexes, processing and maturation of RNA, and trafficking, stabilization, translation, and degradation of mRNA. The interaction between RBP and RNA can emphasize important roles in their respective biological processes. Therefore, rapid and accurate detection of RBP-RNA interactions is essential for studying the functions of RBP and RNA. Herein, we reviewed the basic principles, advantages and disadvantages, and applications of some major RBP-RNA interaction technologies, such as ChIRP (chromatin isolation by RNA purification), CHART (capture hybridization analysis of RNA targets), TriFC (trimolecular fluorescence complementation), RIP (RNA immunoprecipitation), CLIP (UV-crosslinking and immunoprecipitation), RNA Pull-down and RNA EMSA, and aimed to provide new ideas for the discovery of novel technologies.

Key words: RNA binding protein, RNA, RBP-RNA interaction, CLIP