关键词: 肉制品, 掺假, 鉴别, 荧光定量PCR, 芯片

Abstract: In order to establish a rapid chip-based method to identify adulterated mutton, the primers for different animal-sourced components and the reagents required for amplification were freeze-dried and immobilized on blank chip reaction cells beforehand, which could be used in fluorescent quantitative PCR for rapid detection of mutton adulteration. And the performance of prepared chip was evaluated by detection test for simulated adulterated samples, which were made by adding pork, chicken, duck and rat meat into mutton. Compared with ABI7500 fluorescent qPCR, the detection method using rapid chip-based fluorescent qPCR could accurately detect five animal-sourced components, and it had high accuracy and availability. In addition, the time required for PCR amplification of this method was shorter, and it could be easily performed, meeting the requirement for quick detection of mutton adulteration. The preparation of the chip and the establishment of the rapid detection method would effectively simplify the detection process of mutton adulteration and shorten the detection time. Additionally, this method cost lower, and the instruments were easy to carry, which made field testing possible. The research result could provide a strong guarantee for meat safety supervision in China.

Key words: meat product, adulteration, identification, fluorogenic quantitative PCR (real-time PCR), chip