关键词: 微流控芯片, 分泌蛋白, 聚二甲基硅氧烷

Abstract: Microfluidic chips have the features of controllable liquid flow, less sample and reagents consumption, faster analysis speed. It can be used to analyze hundreds of samples in a few minutes or even shorter time, and be sued to realize the sample online pretreatment and analyze the whole process. This paper introduced a barcode microfluidic chip, which with the peculiarity of high density of ssDNA as templates. Traditional protein microfluidic chip had the shortcoming of denaturating easily once fixed on the solid surface. Barcode microfluidic chip not only solved the requirement of stability but also met the requirement of handling large amounts of data. It can be used to detecte lots of cell secretory protein fast. Barcode microfluidic chip is becoming the most attractive analysis tools of detecting secreted proteins, because of its characteristics of simple sample preparation, low consumption and high efficiency and high flux, which has been widely used in the fields of analyzing and detecting sample and clinical research.

Key words: microfluidicss, secreted proteins, PDMS