关键词: 海马, 蛋白, 酶解条件, 优化

Abstract: In order to extract total protein of Hippocampus and optimize conditions for enzymatic hydrolysis, we extracted the total protein of Hippocampus by the method of water extraction of dried Hippocampus. Papain, alkaline protease, neutral protease and trypsin were used respectively under the different pH, time, temperature and E/S conditions to conduct single factor test, and finally the enzymatic hydrolysis conditions were optimized by orthogonal test. The enzymatic hydrolysis condition and protein content under different conditions were determined by SDS-PAGE and BCA, respectively. The optimal enzymolysis conditions were determined according to the degree of proteolysis shown in the charts. The results showed that 1.056 g total protein was extracted from 10 g dried Hippocampus, and its protein concentration was 0.106 g/mL. The best enzyme was alkaline protease. The best enzymatic conditions were pH 9, E/S 5%, hydrolysis temperature 50℃and hydrolysis time 3 h, and the degree of hydrolysis of the Hippocampus protein was 96.9%. The optimal enzymatic hydrolysis conditions have been determined, and the study laid a certain foundation for the study of biological activity of hippocampal polypeptide.

Key words: Hippocampus, protein, hydrolysis conditions, optimization