生物技术进展 ›› 2022, Vol. 12 ›› Issue (6): 825-836.DOI: 10.19586/j.2095-2341.2022.0048

• 进展评述 • 上一篇    下一篇

双分子荧光互补技术在动物病毒中的研究进展

周水娟(), 郭忠建()   

  1. 江苏大学生命科学学院,江苏 镇江 212000
  • 收稿日期:2022-04-02 接受日期:2022-06-30 出版日期:2022-11-25 发布日期:2022-11-30
  • 通讯作者: 郭忠建
  • 作者简介:周水娟 E-mail: 2807948441@qq.com
  • 基金资助:
    国家自然科学基金项目(31770174)

Progress of Bimolecular Fluorescence Complementation Technology in Animal Viruses Research

Shuijuan ZHOU(), Zhongjian GUO()   

  1. Shool of Life Science,Jiangsu University,Jiangsu Zhenjiang 212000,China
  • Received:2022-04-02 Accepted:2022-06-30 Online:2022-11-25 Published:2022-11-30
  • Contact: Zhongjian GUO

摘要:

病毒侵染宿主的过程存在着一系列相互作用,了解病毒与宿主之间的蛋白质相互作用对于深入研究病毒具有重要意义。在众多研究蛋白质相互作用的方法中,双分子荧光互补技术(bimolecular fluorescence complementation,BiFC)因其能在活细胞中可视化相互作用而被广泛应用。介绍了双分子荧光技术的原理、发展和优势,总结了双分子荧光技术在动物病毒以及抗病毒药物研究中的应用,并进一步阐述了新型双分子荧光系统的原理,以期为研究动物病毒致病机制和抗病毒药物研发提供新的思路。

关键词: 双分子荧光互补技术, 蛋白质相互作用, 动物病毒, 抗病毒药物

Abstract:

There are a series of protein-protein interactions in the process of virus infection. Therefore, understanding the interactions between the viruses and the hosts are of great significance for the in-depth studies of virology. Currently, there are many techniques used in protein interactions research, among which the bimolecular fluorescence complementation (BiFC)is widely used due to its ability to visualize interactions in living cells. This paper introduced the principle, development and advantages of BiFC technology. The application of BiFC technology in animal viruses and antiviral drugs were also summarized, and the principle of some new bimolecular fluorescence systems were further elaborated, so as to provide new ideas for the studies of virus pathogenic mechanism and the development of antiviral drugs.

Key words: BiFC, protein-protein interaction, animal viruses, antiviral drugs

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