生物技术进展 ›› 2022, Vol. 12 ›› Issue (4): 623-629.DOI: 10.19586/j.2095-2341.2021.0172

• 研究论文 • 上一篇    下一篇

福鼎大白茶蛋白质相互作用网络研究

王芬(), 潘发莲, 杨秀, 韦永欢, 裴会敏   

  1. 黔南民族师范学院生物科学与农学院,贵州 都匀 558000
  • 收稿日期:2021-10-16 接受日期:2022-01-24 出版日期:2022-07-25 发布日期:2022-08-10
  • 作者简介:王芬 E-mail:fenjiayi521@163.com
  • 基金资助:
    国家自然科学基金项目(31900486);贵州省科技厅基础研究计划项目(黔科合基础〔2019〕1298);贵州省教育厅项目(〔2016〕020;2017347;〔2015〕337;QNYSKYPT2018007;黔南民族师范学院科研创新团队(Qnsyk201605);贵州省植物学重点支持学科(黔学位合字ZDXK〔2016〕23号);黔南民族师范学院项目(QNSY2018ZJ006)

The Protein-protein Interaction Network of Fuding Dabaicha Tea

Fen WANG(), Falian PAN, Xiu YANG, Yonghuan WEI, Huimin PEI   

  1. College of Biological Science and Agriculture,Qiannan Normal University for Nationalities,Guizhou Duyun 558000,China
  • Received:2021-10-16 Accepted:2022-01-24 Online:2022-07-25 Published:2022-08-10

摘要:

蛋白质相互作用网络的构建可以为探究茶树生长过程中的关键蛋白并预测其功能提供理论参考。以贵州都匀地区福鼎大白茶为研究对象,利用三代Nanopore测序技术和同源比对方法构建福鼎大白茶根、茎、叶差异基因蛋白质相互作用网络,通过网络进一步预测关键蛋白及其功能。结果表明,叶与根、叶与茎、茎与根和根茎叶差异基因蛋白质相互作用网络中的关键蛋白分别为53、39、42和53个,并且预测出了关键蛋白的功能,如TEA003744的功能可能为腺苷酸激酶活性、蛋白质丝氨酸/苏氨酸激酶活性和ATP结合,参与光合作用和蛋白质磷酸化过程;TEA026776可能为发育蛋白,参与细胞分化过程和蛋白质磷酸化过程,还具有ATP结合活性和蛋白激酶活性;TEA019056的功能可能为ATP结合、GTP结合和GTP酶活性,参与过氧化物酶体组织的组成和蛋白质磷酸化等。随后预测出4个网络中打分最高功能模块的功能,并进行拓扑属性分析、功能模块分析、集群注释和聚类分析。研究结果可为鉴定蛋白质的功能、寻找关键蛋白及选育优良品种等提供理论依据。

关键词: 福鼎大白茶, 蛋白质相互作用网络, 关键蛋白, 蛋白质功能

Abstract:

The establishment of protein-protein interaction network (PPIN) could provide theoretical reference for exploring the hub proteins and their functions during the growth process of tea trees. Take Fuding Dabaicha as target, We predicted the PPIN via a Interolog method and investigated differential expression genes (DEGs) in roots stems and leaves using Oxford Nanopore technology and blast analysis. We identified the hub proteins from PPIN and predicted their functions. Resuts showed that, there were 53, 39, 42 and 53 hub genes identified as differently expressed in leaf-root, leaf-stem, stem-root and root-stem-leaf respectively. Among them, the functions of TEA003744 was annotated as adenylate kinase activity, protein serine/threonine kinase activity and ATP binding; TEA026776 was a developmental protein, involving in cell differentiation and ATP binding; TEA019056 was annotated as ATP binding, GTP binding and GTPase activity, involving in peroxisome organization. We also performed functional annotation for the highest-scoring module of the four PPINs, and conducted topological attribute analysis, function module analysis, cluster annotation and cluster analysis. The research results provided promising resource and method for developmental essential proteins investigation and tea breeding.

Key words: Fuding Dabaicha, protein-protein interaction network, hub protein, protein function

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