关键词: 香蕉皮提取物, 抗氧化, HepG2细胞, 凋亡

Abstract:

The invitro antioxidant and anticancer potential of banana peel extraction （BPE） against HepG2 cells was evaluated. Quantitative analysis for total phenolic and flavonoid content in BPE were determined by Folin-Ciocalteu and Al（NO₃）₃ colorimetric assay. The antioxidant activity of BPE in vitro was evaluated by measuring the scavenging capacity DPPH and O₂^- free radical. The anticancer effect of the BPE on HepG2 cells was investigated using Alamar Blue assay. The apoptosis of HepG2 cells was detected by fluorescence staining and flow cytometry. Western blot analysis was used to detect the changes of apoptotic proteins. Caspase activity kits were used to determine the activities of Caspase-3 and Caspase-9. The Caspase-9 and Caspase-3 specific inhibitors further validated the signal transduction pathway related to BPE anti-tumor. The total phenolic and flavonoid content in the BPE was 39.23±2.35 mg·L^-1 and 26.53±1.97 mg·L^-1， respectively. The BPE also demonstrated potent DPPH， O₂^- radical scavenging capacity （65.31%±3.82% and 51.29%±4.23%）. BPE significantly inhibited the proliferation of HepG2 cells with an IC₅₀ value of 54.32 mL·L^-1. BPE induced HepG2 apoptosis by up regulating the expression of Caspase-3， Bax， Bid， Apaf-1， Bak， p53 and Caspase-9， and down regulating the Bcl-2 and Bcl-xl. Furthermore， Caspase-9 and Caspase-3 specific inhibitor （Z-LEHD-FMK and Ac-DEVD-CHO） reverse the proliferation inhibitory activity of BPE in HepG2 cells. BPE has a certain free radical scavenging capacity， and has an obvious inhibitory effect on HepG2 cell proliferation， providing data support for further development and utilization of banana peel.

Key words: banana peel extraction, antioxidant, HepG2 cells, apoptosis