关键词: 人慢性髓系白血病K562细胞, LSD1基因, 细胞凋亡, 细胞周期

Abstract: The aim of the study is to uncover the mechanism of reduced proliferation of K562 cells resulting from LSD1 depletion. Making use of the LSD1-/- K562 cell lines, cellular apoptosis was measured by Annexin V/PI double staining with flow cytometry. Cell cycle was examined using PI staining with flow cytometry. We want to explore whether the apoptosis level of K562 cells is altered and whether cell cycle is affected after knockout of LSD1 gene. The results showed that there was no induction of apoptosis in K562 cells after LSD1 depletion; the cell cycle of K562 cells arrested at the G0/G1 phase, cells decline in the DNA duplication phase. The results indicated that LSD1 depletion inhibits K562 cells proliferation mainly via impairment of cell cycle but not apoptosis.

Key words: K562 cells, LSD1 gene, apoptosis, cell cycle