内陆水体盐度升高对小球藻生理特性的影响

doi:10.19586/j.2095-2341.2022.0066

生物技术进展 ›› 2022, Vol. 12 ›› Issue (6): 894-899.DOI: 10.19586/j.2095-2341.2022.0066

内陆水体盐度升高对小球藻生理特性的影响

赵开拓(), 李志龙, 贺达

中冶南方城市建设工程技术有限公司，武汉 430077

收稿日期:2022-04-29 接受日期:2022-06-22 出版日期:2022-11-25 发布日期:2022-11-30
通讯作者: 赵开拓
作者简介:赵开拓 E-mail: 184718324@qq.com。

Effect of Salinity Rising of Inland Water on Physiological Characteristics of Chlorella vulgaris

Kaituo ZHAO(), Zhilong LI, Da HE

WISDRI City Construction Engineering & Research Incorporation Ltd. ，Wuhan 430077，China

Received:2022-04-29 Accepted:2022-06-22 Online:2022-11-25 Published:2022-11-30
Contact: Kaituo ZHAO

摘要/Abstract

摘要：

全球气候变化引起内陆水体盐度缓慢升高，对浮游植物造成显著影响。为了探究水体盐度升高对浮游植物生理特性的影响，以普通小球藻为试验对象，在原水和不同盐度（1、3、5 g·L^-1）条件下研究普通小球藻生长增殖情况，分析其抗氧化酶活性及对光合作用的生理响应。结果显示，盐度升高会抑制普通小球藻生长，当达到3 g·L^-1以上时抑制作用明显，细胞分裂增殖指标均明显下降，叶绿素a合成速率衰减，藻细胞膜损坏严重，同时其光合活性显著降低。这表明过高盐度（>3 g·L^-1）极有可能破坏藻细胞的生理结构和抗氧化能力，导致其生长增殖受到抑制。研究结果从细胞增殖和生理特性角度分析了水体盐度变化对水体浮游藻类的影响，以期为保护内陆水体生物多样性提供参考。

关键词: 盐度升高, 小球藻, 抗氧化指标, 光合活性

Abstract:

With the change of global climate， the problem of salinity slowly rising of inland water has a remarkable effect on the phytoplankton. To investigate the effect on physiological property of freshwater phytoplankton under the increasing salty environment， Chlorella vulgaris was taken as the research object and cultured at raw water and salinity（1， 3， 5 g·L^-1）. The effect of low salt level on growth and multiplication of Chlorella vulgaris was studied and its physiological response such as antioxidant enzymatic activity and photosynthesis were analyzed. The results showed that， with the rising of salinity， the growth of Chlorella vulgaris slowed down， the generating rate of chlorophyll-a decreased， its membrane structure was badly damaged， and the photosynthetic activity markedly decreased. All the results showed that high salinity （>3 g·L^-1） very likely damaged cells physiological structure and enzyme system of algae cells so that inhibited its growth and multiplication. The effects of salinity changes on planktonic algae were studied from the perspective of cell proliferation and physiological characteristics， which was expected to provide reference for protecting the biodiversity of inland water.

Key words: rising salinity, Chlorella vulgaris, antioxidant index, photosynthetic activity

中图分类号:

Q949.2

赵开拓, 李志龙, 贺达. 内陆水体盐度升高对小球藻生理特性的影响[J]. 生物技术进展, 2022, 12(6): 894-899.

Kaituo ZHAO, Zhilong LI, Da HE. Effect of Salinity Rising of Inland Water on Physiological Characteristics of Chlorella vulgaris[J]. Current Biotechnology, 2022, 12(6): 894-899.

图/表 8

图1 不同盐度下的细胞数及细胞分裂图A：对照组；B：盐度1 g·L-1；C：盐度3 g·L-1；D：盐度5 g·L-1

Fig. 1 Cells amount and division in different salinity

表1 不同盐度下普通小球藻细胞相对分裂比例

Table 1 Divide ratio of Chlorella vulgaris cells under different salinity

组别	相对分裂比例（%）
对照组	77.0
1 g·L^-1实验组	33.0
3 g·L^-1实验组	18.0
5 g·L^-1实验组	1.1

图2 试验期末处理组叶绿素a的最终浓度注：**表示不同处理组与1 g·L-1实验组相比在P<0.01水平具有统计学差异。

Fig. 2 Comparison of final concentration of chlorophyll-a

图3 不同盐度下叶绿素a浓度变化过程

Fig. 3 Change of chlorophyll-a concentration in different salinity

图4 不同盐度下MDA平均含量变化注：*表示不同处理组与对照组相比在P<0.05水平具有统计学差异。

Fig. 4 Average content of MDA under different salinity

图5 不同盐度下SOD酶比活性变化

Fig. 5 The change of SOD specific activity under different salinity

图6 不同盐度下光化学淬灭系数qP的变化

Fig. 6 Change of photochemical quenching coefficient qP under different salinity

图7 不同盐度下非光化学淬灭系数NPQ的变化

Fig. 7 Change of non-photochemical quenching coefficient NPQ under different salinity

参考文献 23

1	ENID L L. Global climate change impacts in the united states: a state of knowledge report from the U.S. global change research program[J]. J. Geogr., 2013, 112(4): 178.
2	THOMPSON P A, O'BRIEN T D, PAERL H W, et al.. Precipitation as a driver of phytoplankton ecology in coastal waters: a climatic perspective[J]. Estuar. Coast. Shelf S., 2015, 162(1): 119-129.
3	NIELSEN D L, BROCK M A. Modified water regime and salinity as a consequence of climate change: prospects for wetlands of Southern Australia[J]. Climatic Change, 2009, 95(3-4): 523-533.
4	NIELSEN D L, BROCK M A, REES G N, et al.. Effects of increasing salinity on freshwater ecosystems in Australia[J]. Aust. J. Bot., 2003, 51(6): 655-665.
5	王效科.中国西北湿地群干旱之地的温润[J].森林与人类, 2010, 1(8): 10-23.
6	周洪华,李卫红,陈亚宁,等.博斯腾湖水盐动态变化(1951-2011年)及对气候变化的响应[J].湖泊科学, 2014, 26(1): 55-65.
7	WILLIAMS W D. Salinisation: a major threat to water resources in the arid and semi-arid regions of the world[J]. Lakes Reserv., 1999, 4(3-4): 85-91.
8	NIELSEN D L, BROCK M A, VOGEL M, et al.. From fresh to saline: a comparison of zooplankton and plant communities developing under a gradient of salinity with communities developing under constant salinity level[J]. Mar. Freshwater Res., 2008, 59(7): 549-559.
9	杜宇,孙雪,徐年军.不同盐度和Fe~(3+)浓度对小球藻生长、硝酸还原酶活性及基因表达的影响[J].生态科学, 2012, 31(4): 441-445.
10	周连宁,吴锋,赵振业,等.重要环境因子对小球藻去除污水中氮磷的影响[J].生物技术进展, 2015, 5(1): 60-65.
11	刘春光,孙红文,朱琳,等.两种无机盐形成的盐度对淡水藻类生长的影响[J].环境科学学报, 2006, 26(1): 157-161.
12	饶本强,张列宇,吴沛沛,等.集球藻对盐胁迫的生理适应及细胞结构变化[J].水生生物学报, 2012, 36(2): 329-338.
13	葸玉琴,马春林,曹云涛,等.自养和混养小球藻对NaCl胁迫的生理响应[J].生物技术进展, 2013, 3(3): 223-228.
14	梁兴飞,郭宗楼.超声辅助热乙醇提取法测定浮游植物叶绿素a的方法优化[J].水生生物学报, 2010, 34(4): 856-861.
15	李合生.植物生理生化实验原理和技术[M].北京: 高等教育出版社, 2000.
16	高俊凤. 植物生理学实验指导[M].北京: 高等教育出版社, 2006.
17	LI Y, ZHANG Y P, SUN M, et al.. Research advance in the effects of salt stress on plant and the mechanism of plant resistance[J]. Chin. Agric. Sci. Bull., 2008, 24(1): 258-265.
18	ISMAIEL M M S, EL-AYOUTY Y M, PIERCEY-NORMORE M. Role of pH on antioxidants production by Spirulina (Arthrospira) platensis [J]. Braz. J. Microbiol., 2016, 47(2): 298-304.
19	吴赛君,周香君,赵剑,等.镍和砷对铜绿微囊藻光合效应的影响[J].环境科学与技术, 2015, 38(10): 27-31.
20	任佳佳,洪廷,张宁,等.盐度对波吉卵囊藻叶绿素荧光参数的影响及PsbA基因的克隆与表达分析[J].广东海洋大学学报, 2020, 40(3): 30-39.
21	张国伟,张雷,唐明星,等.土壤盐分对棉花功能叶气体交换参数和叶绿素荧光参数日变化的影响[J].应用生态学报, 2011, 22(7): 1771-1781.
22	CHOUDHARY M, JETLEY U K, ABASH K M, et al.. Effect of heavy metal stress on proline, malondialdehyde, and superoxide dismutase activity in the cyanobacterium Spirulina platensis-S5[J]. Ecotox. Environ. Safe, 2007, 66(2): 204-209.
23	孙妮,曹广霞,彭娟.盐胁迫对栅藻SP-01抗氧化酶和代谢产物的影响[J].南方农业学报, 2014,45(9): 15-20.

[1]	周连宁,吴锋,赵振业,王波. 重要环境因子对小球藻去除污水中氮磷的影响[J]. 生物技术进展, 2015, 5(1): 60-65.
[2]	葸玉琴,马春林,曹云涛,孔维宝,杨红. 自养和混养小球藻对NaCl胁迫的生理响应[J]. 生物技术进展, 2013, 3(3): 223-228.

内陆水体盐度升高对小球藻生理特性的影响

Effect of Salinity Rising of Inland Water on Physiological Characteristics of Chlorella vulgaris

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