关键词: 非小细胞肺癌, KIF26B, 迁移, 侵袭, 凋亡, 周期

Abstract: Although kinesin is related to tumorigenesis closely, whether it is related to the expression and development of non-small lung cancer, and its specific biological effects and the molecular mechanisms have been few reported yet. After interfering with KIF26B, we explored the effects on proliferation, invasion, migration, cell cycle, apoptosis, and expression of related proteins in non-small cell lung cancer. By analyzing the mRNA TCGA database information, it was concluded that KIF26B gene is highly expressed in non-small cell lung cancer. Then the expression level of KIF26B in several common non-small cell lung cancer cell lines was detected by qRT-PCR, and KIF26B was screened for high expression in A549 and NCI-H292 cell lines. After using RNA interference (siRNA) to silence the KIF26B gene of A549 and NCI-H292 cells, the silenced KIF26B gene was detected by CCK8, real time cell analyzer (RTCA), plate cloning and Transwell experiments. Flow cytometry was used to analyze the effects of silenced KIF26B gene on cell cycle and apoptosis in NCI-H292 and A549, and Western blotting was used to detect the protein expression. The results showed that after silenced KIF26B, the proliferation of A549 and NCI-H292 cells was significantly reduced, and the ability to invade and migrate was significantly reduced. After silencing KIF26B, A549 and NCI-H292 cells were prevented from transforming from G1 to S phase, and apoptotic cells were significantly increased. Besides, the protein expression level of related cyclin D1, Bcl-2, E-cadherin and Vimentin reduced significantly, while activated Caspase-3 (active Caspase-3) and its cleavage substrate PARP1 (cleaved PARP1) expression levels were significantly up-regulated. The results indicated that KIF26B may be a tumor-promoting gene for non-small cell lung cancer, and it is involved in the occurrence and development of non-small cell lung cancer, KIF26B is expected  to be a potential target for the treatment of NSCLC.

Key words: non-small cell lung cancer（NSCLC）, KIF26B, migration, invasion, apoptosis, cell cycle