关键词: 氢分子, 肝癌, 肿瘤防治

Abstract: The effects of molecular hydrogen on various diseases such as cancer are predictable. It is simple to obtain, easy to use and free of side effects. In order to clarify the effect of molecular hydrogen on hepatoma cell Huh7, the effect of molecular hydrogen on Huh7 cells was studied from the aspects of cell viability, cell cycle and apoptosis, cell neoplasm formation, cell migration and invasion. The results of cell viability test (CCK-8 method) showed that molecular hydrogen had obvious inhibitory effect on Huh7 cell activity. In the experiment of cell neoplasm formation, the clone ball experiment showed that the number of cell globules in the hydrogen-containing medium group (H) decreased, while the diameter of clone ball decreased significantly (P<0.000 1). The plate cloning experiment showed that the number of cell clones in group H also decreased significantly (P<0001), indicating that molecular hydrogen can inhibit the dryness of Huh7 cells. Molecular hydrogen also had obvious effects on Huh7 cell cycle, and had the effects of promoting apoptosis and inhibiting cell migration and infiltration. At the same time, hydrogen molecules could also inhibit the expression of vimentin in cells. Studies showed that hydrogen molecules reduced the activity and proliferation ability of Huh7 cells, weakened the dryness of Huh7 cells, inhibited the cell invasion and migration ability, and reduced the expression of cellular vimentin, which provided a certain experimental basis for the application of hydrogen molecules in the prevention and treatment of liver cancer.

Key words: molecular hydrogen, liver cancer, cancer prevention and treatment