关键词: 氢分子, 宫颈癌, 肿瘤防治

Abstract: The effects of molecular hydrogen on various diseases are predictable. It is easy to use without side effects. Many results also prove that molecular hydrogen is effective on the prevention and treatment of tumors. The effects of molecular hydrogen on HeLa cells were determined in terms of cell proliferation, cell neoplasm formation, viability, cell cycle, apoptosis, migration and invasion. The results showed that hydrogen treatment led to a marked reduction of sphere number and average diameter in HeLa cells. And hydrogen treatment induced markedly fewer and smaller colonies in HeLa cells. Hydrogen treatment induced in a slightly slower growth rate in HeLa cells. And it had a certain role in promoting the expression of vimentin in HeLa cells. In addition, the effect of molecular hydrogen on HeLa cell cycle was significant. In parallel, a matrigel invasion assay was also performed to determine the effect of hydrogen treatment on the invasion of HeLa cells. Wounding assay was employed to examine the influence of hydrogen treatment on the migration of human ovarian cancer cells. The presence of hydrogen significantly inhibited the motile nature of HeLa cells. The results showed that molecular hydrogen promoted the expression of vimentin and decreased the proliferation rate of cells, but inhibited the invasion and migration of HeLa cells. It provides a certain experimental basis for the prevention and treatment of cervical cancer.

Key words: molecular hydrogen, cervical cancer, cancer prevention and treatment