关键词: 表面等离子体共振, 电化学, 生物传感器, DNA

Abstract:

Surface plasmon resonance (SPR) is designed to detect the change of refractive index near the surface. It is characterized by unmarked, real-time, sensitive and fast. It is often used to study molecular interactions, including kinetics, efficiency constant and macromolecular conformational change and so on. Electrochemical (electrochemical, EC) technology is a mature technology for qualitative and quantitative study of electron transfer, redox and interfacial adsorption. It has the advantages of simplicity, low cost and miniaturization of equipment. The existing DNA hybridization techniques, such as optical, electrochemical or piezoelectric transduction techniques, mainly focus on improving the selectivity and sensitivity of DNA hybridization detection system. In the area of DNA analysis, the traditional SPR is limited in the application of ultra-sensitive detection because it is unable to measure the minimal change of refractive index. Therefore, with the development of nanomaterials and the rapid development of hyphenated technology, the research of biosensors combined with SPR and EC has become the focus with more and more attention. In recent years, there have been few report on the combination of SPR and EC in the detection of DNA. Therefore, this paper briefly introduced the technical principle, hyphenated methods and application progress of SPR and EC detection of DNA, which was expected to provide reference for the research of DNA sensors based on surface plasmon resonance and electrochemical combination.

Key words: surface plasmon resonance, electrochemical, biosensors, DNA