生物技术进展 ›› 2022, Vol. 12 ›› Issue (1): 1-9.DOI: 10.19586/j.2095-2341.2021.0109
• 进展评述 • 下一篇
收稿日期:
2021-06-15
接受日期:
2021-08-05
出版日期:
2022-01-25
发布日期:
2022-01-26
通讯作者:
王凯
作者简介:
张会 E-mail:hzhangfly107@126.com;
基金资助:
Received:
2021-06-15
Accepted:
2021-08-05
Online:
2022-01-25
Published:
2022-01-26
Contact:
Kai WANG
摘要:
着丝粒(centromere)是真核生物染色体的重要功能结构。在细胞有丝分裂和减数分裂过程中,着丝粒通过招募动粒蛋白行使功能,保障染色体正确分离和传递。真核生物中,含有着丝粒特异组蛋白的CenH3区域被定义为功能着丝粒区,即真正意义上的着丝粒。近年来,借助染色质免疫沉淀技术,人们对功能着丝粒DNA开展了深入研究,揭示其组成、结构及演化特征,并发现功能着丝粒区存在具有转录活性的基因,且部分基因具有重要生物学功能。由于存在大量重复DNA,着丝粒演化之谜一直未能完全揭示。对植物功能着丝粒DNA序列研究进展进行了概述,并重点阐述了着丝粒重复DNA研究的新方法和新进展,以期为深入开展相关研究提供借鉴。
中图分类号:
张会, 王凯. 植物功能着丝粒DNA研究进展[J]. 生物技术进展, 2022, 12(1): 1-9.
Hui ZHANG, Kai WANG. The Research Progress on Plant Functional Centromere DNAs[J]. Current Biotechnology, 2022, 12(1): 1-9.
属名 | 种名 | 重复序列 | 序列大小/bp |
---|---|---|---|
拟南芥属(Arabidopsis) | 大沙叶芥(A. arenosa) | pAa214 | 166~179 |
叶芽鼠耳芥(A. gemmifera) | pAge1,pAge2 | 180 | |
无苞芥(A. pumila) | pAgKB2,pAgKB1 | 180 | |
鼠耳芥(A. thaliana) | pAL1 | 180 | |
落花生属(Arachis) | A. batizocoi | AbaTR⁃2 | 297 |
落花生(A. hypogaea) | Cot⁃1⁃5,Cot⁃1⁃59,Cot⁃1⁃119 | 115 | |
A. helodes | AheTR⁃2 | 295 | |
A. trinitensis | AtrTR⁃2 | 300 | |
甜菜属(Beta) | 白花甜菜(B. corolliflora) | pHC8 | 162 |
平匍甜菜(B. procumbens) | pTS5,pTS4.1 | 158~312 | |
栽培甜菜(B. vulgaris) | pBv1 | 326~327 | |
芸薹属(Brassica) | 紫菜薹(B. campestris) | pBcKB4,pBT11 | 175 |
结球甘蓝(B. oleracea) | pBoKB1 | 171 | |
稻属(Oryza) | 短花药野生稻(O. brachyantha) | CentO⁃F | 154 |
根茎野生稻(O. rhizomatis) | CentO⁃C1,CentO⁃C2 | 126,366 | |
栽培稻(O. sativa) | CentO | 155 | |
茄属(Solanum) | S. bulbocastanum | pSbTC1 | 7 |
马铃薯(S. tuberosum) | St18,St24,St49,St57,St3⁃58,St3⁃238,St3⁃294 | 979~5 390 | |
紫云英(Astragalus sinicus) | CentAs | 20 | |
二穗短柄草(Brachypodium distachyon) | CentBd | 156 | |
大豆[Glycine max (soybean)] | CentGm⁃1,CentGm⁃2,CentGm⁃4 | 91~411 | |
光叶百脉根(Lotus japonicus) | Ljcen1 | 440 | |
紫苜蓿(Medicago sativa) | MsCR | 15~168 | |
御谷(Pennisetum glaucum) | pPgKB19 | 137 | |
甘蔗热带种(Saccharum officinarum) | SCEN | 140 | |
高粱[Sorghum bicolor (sorghum)] | pSau3A10 | 137 | |
小麦[Triticum aestivum (wheat)] | Tail family | 570 | |
三叶草(Trifolium repens) | TrR350 | 350 | |
豇豆(Vigna unguiculata) | pVuKB1 | 488 | |
玉米(Zea mays) | CentC,Cent4 | 156,740 |
表1 植物中已知的着丝粒串联重复序列[23]
Table 1 Known centromeric tandem repeats in plants[23]
属名 | 种名 | 重复序列 | 序列大小/bp |
---|---|---|---|
拟南芥属(Arabidopsis) | 大沙叶芥(A. arenosa) | pAa214 | 166~179 |
叶芽鼠耳芥(A. gemmifera) | pAge1,pAge2 | 180 | |
无苞芥(A. pumila) | pAgKB2,pAgKB1 | 180 | |
鼠耳芥(A. thaliana) | pAL1 | 180 | |
落花生属(Arachis) | A. batizocoi | AbaTR⁃2 | 297 |
落花生(A. hypogaea) | Cot⁃1⁃5,Cot⁃1⁃59,Cot⁃1⁃119 | 115 | |
A. helodes | AheTR⁃2 | 295 | |
A. trinitensis | AtrTR⁃2 | 300 | |
甜菜属(Beta) | 白花甜菜(B. corolliflora) | pHC8 | 162 |
平匍甜菜(B. procumbens) | pTS5,pTS4.1 | 158~312 | |
栽培甜菜(B. vulgaris) | pBv1 | 326~327 | |
芸薹属(Brassica) | 紫菜薹(B. campestris) | pBcKB4,pBT11 | 175 |
结球甘蓝(B. oleracea) | pBoKB1 | 171 | |
稻属(Oryza) | 短花药野生稻(O. brachyantha) | CentO⁃F | 154 |
根茎野生稻(O. rhizomatis) | CentO⁃C1,CentO⁃C2 | 126,366 | |
栽培稻(O. sativa) | CentO | 155 | |
茄属(Solanum) | S. bulbocastanum | pSbTC1 | 7 |
马铃薯(S. tuberosum) | St18,St24,St49,St57,St3⁃58,St3⁃238,St3⁃294 | 979~5 390 | |
紫云英(Astragalus sinicus) | CentAs | 20 | |
二穗短柄草(Brachypodium distachyon) | CentBd | 156 | |
大豆[Glycine max (soybean)] | CentGm⁃1,CentGm⁃2,CentGm⁃4 | 91~411 | |
光叶百脉根(Lotus japonicus) | Ljcen1 | 440 | |
紫苜蓿(Medicago sativa) | MsCR | 15~168 | |
御谷(Pennisetum glaucum) | pPgKB19 | 137 | |
甘蔗热带种(Saccharum officinarum) | SCEN | 140 | |
高粱[Sorghum bicolor (sorghum)] | pSau3A10 | 137 | |
小麦[Triticum aestivum (wheat)] | Tail family | 570 | |
三叶草(Trifolium repens) | TrR350 | 350 | |
豇豆(Vigna unguiculata) | pVuKB1 | 488 | |
玉米(Zea mays) | CentC,Cent4 | 156,740 |
种名 | 重复序列 | 序列大小/bp |
---|---|---|
拟南芥(Arabidopsis thaliana) | Athila | 10 500 |
落花生(Arachis hypogaea) | Cot⁃1⁃Clone117(partial) | 117 |
平匍甜菜(Beta procumbens) | pBp10 | 417 |
甜菜(Beta vulgaris) | pBv26 | 417 |
二穗短柄草(Brachypodium distachyon) | CRBd1 | — |
大豆[Glycine max (soybean)] | GmCR | 421~852 |
雷蒙德棉(Gossypium raimondii) | Gr087,Gr201,Gr285,Gr291,Gr298,Gr304,Gr327,Gr334,Gr344,Gr359,Gr369,Gr372,Gr386,Gr407,Gr442,Gr452,Gr471,Gr552,Gr616 | 309~5 909 |
大麦(Hordeum vulgare) | cereba | 7 176 |
番茄(Lycopersicum esculentum) | TGRIV | 7 000 |
水稻(Oryza sativa) | CRR | 7 400~7 800 |
黑麦(Secale cereale) | Bilby | 3 400 |
甘蔗热带种(Saccharum officinarum) | CRS | 3 600 |
高粱(Sorghum bicolor) | pSau3A9 | 745 |
野生一粒小麦(Triticum boeoticum) | CRWs | 7 762~7 865 |
玉米(Zea mays) | CRM | 7 572 |
Zingeria biebersteiniana | Zb47A | 263 |
表2 植物着丝粒特异反转录转座子序列[23]
Table 2 The centromeric retrotransposons in plants[23]
种名 | 重复序列 | 序列大小/bp |
---|---|---|
拟南芥(Arabidopsis thaliana) | Athila | 10 500 |
落花生(Arachis hypogaea) | Cot⁃1⁃Clone117(partial) | 117 |
平匍甜菜(Beta procumbens) | pBp10 | 417 |
甜菜(Beta vulgaris) | pBv26 | 417 |
二穗短柄草(Brachypodium distachyon) | CRBd1 | — |
大豆[Glycine max (soybean)] | GmCR | 421~852 |
雷蒙德棉(Gossypium raimondii) | Gr087,Gr201,Gr285,Gr291,Gr298,Gr304,Gr327,Gr334,Gr344,Gr359,Gr369,Gr372,Gr386,Gr407,Gr442,Gr452,Gr471,Gr552,Gr616 | 309~5 909 |
大麦(Hordeum vulgare) | cereba | 7 176 |
番茄(Lycopersicum esculentum) | TGRIV | 7 000 |
水稻(Oryza sativa) | CRR | 7 400~7 800 |
黑麦(Secale cereale) | Bilby | 3 400 |
甘蔗热带种(Saccharum officinarum) | CRS | 3 600 |
高粱(Sorghum bicolor) | pSau3A9 | 745 |
野生一粒小麦(Triticum boeoticum) | CRWs | 7 762~7 865 |
玉米(Zea mays) | CRM | 7 572 |
Zingeria biebersteiniana | Zb47A | 263 |
图2 基于体外环化机制的着丝粒串联重复DNA演化模式注:含有末端重复序列(红色三角形)的着丝粒反转录转座子(a)通过染色体内同源重组或者小片段同源末端连接修复等机制(b)形成游离于染色体外的环化DNA(c);环化DNA可经过环化扩增,形成不同长度串联重复序列(d);并通过同源重组等机制再整合到染色体上的着丝粒区(e)。
Fig 2 The proposed eccDNA?based on rolling circle amplification mechanism.
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