关键词: 杏, 蔗糖转运蛋白基因, 生物信息学

Abstract: Sucrose is transported into fruit cells by sucrose transporters in fruit trees. The transport efficiency of sucrose transporters affects the sugar content and  quality of fruits. Reverse transcript PCR technology was used in this study. Prunus armeniaca sucrose transport protein gene SUC4 (PaSUC4) was obtained from Jinong red apricot. The full cDNA was 2 480 bp according to the sequencing result. The codon region was 1 500 bp, encoding 499 amino acids. The protein molecular weight was 53.58 kDa, the soelectric point was 9.31. Results of similarity and phylogenetic analysis showed that the sucrose transporters we got had high similarity with that in Prunus (Rosaceae), speculating the function was also conserved. The expression level of PaSUC4 was highest in mature leaves and lowest in pistils. The characteristics of predicted protein had been analyzed by bioinformatics software. For further verification of the gene biological function, the expression vector had been constructed and transformed into tobacco plants. The research results was expected to provide reference for apricot breeding.

Key words: