黑曲霉降解人参皂苷Rb1制备稀有皂苷Compound K

doi:10.3969/j.issn.2095-2341.2016.02.04

生物技术进展 ›› 2016, Vol. 6 ›› Issue (2): 98-104.DOI: 10.3969/j.issn.2095-2341.2016.02.04

黑曲霉降解人参皂苷Rb1制备稀有皂苷Compound K

高娟1,周安东2,原野1,赵天蛟1

1.东北师范大学生命科学学院, 长春130024;

2.东北师范大学附属中学, 长春 130024

收稿日期:2015-12-15 出版日期:2016-03-25 发布日期:2016-01-06
作者简介:高娟，讲师，主要从事糖类化合物生物转化研究。E-mail：gaoj199@nenu.edu.cn
基金资助:
国家自然科学基金（31400299）资助。

Enzymatic Degradation of Ginsenoside Rb1 for Preparation of Compound K by Aspergillus niger sp. J7

GAO Juan, ZHOU An-dong, YUAN Ye, ZHAO Tian- jiao

1.School of Life Sciences, Northeast Normal

University, Changchun 130024, China;

2.High School Attached to Northeast Normal

University, Changchun 130024, China

Received:2015-12-15 Online:2016-03-25 Published:2016-01-06

摘要/Abstract

摘要： 稀有人参皂苷Compound K（C-K）具有显著的生物学活性。从东北农耕土壤中分离出9株真菌，系统研究了其转化人参皂苷Rb1制备C-K的能力。其中，黑曲霉Aspergillus niger sp. J7能够高效转化人参皂苷Rb1生成C-K，转化途径为Rb1→Rd→F2→C-K。对黑曲霉J7转化Rb1制备C-K 的条件进行优化，在最优条件下，Rb1可完全转化成C-K 。将该转化体系扩大到200 mL，60 h内可将Rb1完全转化成C-K，转化率为74.7%。黑曲霉J7为人参皂苷Rb1高效水解为稀有人参皂苷C-K奠定了基础。

关键词: 人参皂苷, 黑曲霉, 生物转化, &beta, -葡萄糖苷酶

Abstract: Minor ginsenoside Compound K (C-K) exhibited remarkable biological activities. In this study, nine fungi were isolated from soil, and their abilities of transform ginsenoside Rb1 for preparation of C-K were studied. A highly efficient ginsenoside Rb1-hydrolyzing fungus Aspergillus niger sp. J7 was screened. The strain converted ginsenoside Rb1 to C-K with the pathway of Rb1→Rd→F2→C-K. The conversion conditions of A. niger sp. J7 was optimized. Under the optimal conditions, ginsenoside Rb1 was converted to C-K completely. The transformation system was enlarged to 200 mL, in which ginsenoside Rb1 was completely converted into C-K after 60 h and the conversion rate was 74.7%. This fungus was expected to be the basis in bioactive C-K preparation.

Key words:

ginsenoside, Aspergillus niger, biotransformation;β-glucosidase

高娟,周安东,原野,赵天蛟. 黑曲霉降解人参皂苷Rb1制备稀有皂苷Compound K[J]. 生物技术进展, 2016, 6(2): 98-104.

GAO Juan1, ZHOU An-dong2, YUAN Ye1, ZHAO Tian-. Enzymatic Degradation of Ginsenoside Rb1 for Preparation of Compound K by Aspergillus niger sp. J7[J]. Curr. Biotech., 2016, 6(2): 98-104.

[1]	陈晓光, 潘晓峰, 王帆, 潘宋斌. 人参皂苷Rg1对阿尔茨海默症大鼠BDNF⁃TrkB信号通路的影响[J]. 生物技术进展, 2022, 12(3): 446-451.
[2]	陈宝莉,秦臻,赵黎明. 粪肠球菌β-N-乙酰氨基葡萄糖苷酶基因的克隆表达及酶学性质表征[J]. 生物技术进展, 2019, 9(3): 296-302.
[3]	郭枫,刘焕焕,邱智东,王伟楠. 稀有人参皂苷的酶转化研究进展[J]. 生物技术进展, 2017, 7(4): 279-283.
[4]	李杰,张陈,张维,周正富. 基于β-半乳糖苷酶为报告基因的耐辐射异常球菌启动子检测载体构建[J]. 生物技术进展, 2016, 6(4): 265-270.
[5]	高秀君,闫培生,刘欣,宝钲,朱延平. 鸡油菌生物转化虾加工废弃物生产抗氧化胞外多糖的研究(英文)[J]. 生物技术进展, 2015, 5(3): 223-234.
[6]	周生民,赵伟,冯文红,段莹莹,曹大鹏. 葡萄糖酸钠发酵过程中对pH细化控制的研究[J]. 生物技术进展, 2014, 4(4): 296-298.
[7]	李书涛,樊攀,程景伟,杨雪鹏. 肠膜明串珠菌α-葡萄糖苷酶基因克隆表达及酶学性质[J]. 生物技术进展, 2012, 2(2): 124-129.
[8]	李明,赵卉. 胚胎干细胞移植治疗糖尿病的研究进展[J]. 生物技术进展, 2011, 1(3): 201-206.

黑曲霉降解人参皂苷Rb1制备稀有皂苷Compound K

Enzymatic Degradation of Ginsenoside Rb1 for Preparation of Compound K by Aspergillus niger sp. J7

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 8

编辑推荐

Metrics

本文评价