关键词: &beta, -半乳糖苷酶；启动子活性检测；耐辐射异常球菌；穿梭质粒pRADZ1

Abstract: Deinococcus radiodurans is a robust bacterium which shows extreme ionizing-radiation tolerance. Abundant proteins and transcriptional factors are important for the various stress response in D. radiodurans. In order to construct the promoter function analysis system that use β-galactosidase gene as report gene in D. radiodurans, we used the genome of D. radiodurans as template to amplificate the promoters of groEL, hsp20, relA and relQ by PCR, then connected them to E. coli-D. radiodurans shuttle vector pRADZ1, obtained the recombinant plasmids pRADZ-PgroEL, pRADZ-Phsp20, pRADZ-PrelA and pRADZ-PrelQ, which could analyze the activity of promoter. Then we transformed those recombinant plasmids into D. radiodurans, and measured the activity of β-galactosidase, the result showed that these promoters can start expression of Lac-Z, and could be regulated under stress. This study provided theoretical basis for the further work of stress response mechanism in Deinococcus radiodurans.

Key words: β-galactosidase, promoter function analysis, Deinococous radiodurans, shuttle vector