生物技术进展 ›› 2016, Vol. 6 ›› Issue (1): 41-46.DOI: 10.3969/j.issn.2095-2341.2016.01.09

• 研究论文 • 上一篇    下一篇

铜绿假单胞菌BRC-CXG5菌株的分离鉴定及其对松墨天牛(Monochamus alternatus Hope)幼虫的杀虫活性

牟亚妮1,徐卓吟2,翁小倩2,黄志成2,任文琳2,吴松青3*   

  1. 1.福建农林大学生命科学学院, 福州 350002;
    2.福建农林大学, 生物农药与化学生物学教育部重点实验室, 福州 350002;
    3.福建农林大学林学院, 福州 350002
  • 收稿日期:2015-11-24 修回日期:2015-12-15 出版日期:2016-01-25 发布日期:2016-03-31
  • 通讯作者: 吴松青,讲师,博士,主要从事微生物农药研究。E-mail:dabinyang@126.com
  • 作者简介:牟亚妮,本科生,主要从事微生物农药研究。E-mail:350744415@qq.com。
  • 基金资助:

    国家林业公益性行业科研专项(201304401);福建农林大学重点项目建设专项(6112C035003);国家大学生创新创业计划项目(201510389025;201510389028);福建省大学生创新创业训练计划项目(201510389101)资助。

Characterization and Identification of Pseudomonas aeruginosa Strain BRC-CXG5 and its Toxicity to Monochamus alternatus Hope Larva

MOU Ya-ni, XU Zhuo-yin, WENG Xiao-qian, HUANG Zhi-cheng, REN Wen-lin, WU Song-qing   

  1. 1.College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
    2.Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
    3.Forestry College, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Received:2015-11-24 Revised:2015-12-15 Online:2016-01-25 Published:2016-03-31

摘要: 从松墨天牛(Monochamus alternatus Hope)虫尸中分离获得一株对其有毒力作用的菌株BRC-CXG5,经培养特征观察、形态学鉴定、生理生化指标测定、16S rDNA碱基序列测定和同源性分析、药敏分析以及生物测定,鉴定该菌株属于铜绿假单胞菌属(Pseudomonas aeruginosa)。研究发现,该菌对一些常见药物具有不同程度的敏感性,对松墨天牛幼虫具有杀虫活性。研究结果可为开发新的微生物农药提供材料和理论基础。  

关键词: 铜绿假单胞菌, 杀虫活性, 分离鉴定, 松墨天牛

Abstract: Pseudomonas aeruginosa strain BRC-CXG5, with cytotoxic activity to Monochamus alternatus Hope larva, was isolated from dead larva. The strain was identified by morphological observation, biochemical and physiological characterization and 16S rDNA sequence analysis, results indicated that BRC-CXG5 was affiliated to Pseudomonas sp.. The strain BRC-CXG5 was identified as Pseudomonas aeruginosa, combined with the 16S rDNA sequence analysis. BRC-CXG5 showed high cytotoxic activity to Monochamus alternatus Hope larva based on bioassay data. The results provided a material and theoretical base for the effective development of biological pest control agents.

Key words: Pseudomonas aeruginosa, insecticidal activity, characterization and identification, Monochamus alternatus Hope