生物技术进展 ›› 2014, Vol. 4 ›› Issue (1): 22-29.DOI: 10.3969/j.issn.2095-2341.2014.01.05.

• 研究论文 • 上一篇    下一篇

玉米精氨酸甲基转移酶蛋白家族生物信息学分析

钱叶雄,徐士杰,张亚男,张梦飞   

  1. 安徽师范大学生命科学学院, 安徽 芜湖 241000
  • 收稿日期:2013-09-13 出版日期:2014-01-25 发布日期:2013-11-04
  • 作者简介:钱叶雄,副教授,博士,研究方向为玉米表观遗传学与生物信息学。E-mail:qyx2011@mail.ahnu.edu.cn
  • 基金资助:

    中国博士后科学基金项目(2012M521212);安徽省自然科学基金项目(1308085MC44);安徽省高校省级自然科学研究重点项目(KJ2013A132)资助。

Bioinformatics Analysis of PRMT Protein Family in Maize

QIAN Ye-xiong, XU Shi-jie, ZHANG Ya-nan, ZHANG Meng-fei   

  1. College of Life Sciences, Anhui Normal University, Anhui Wuhu 241000, China
  • Received:2013-09-13 Online:2014-01-25 Published:2013-11-04

摘要: 蛋白质精氨酸甲基转移酶(protein arginine methyltransferases, PRMTs)是真核生物基因组中重要的表观遗传调控因子之一,主要参与组蛋白精氨酸位点甲基化修饰,改变真核基因组的染色质结构,对基因的表达进行调控。本研究鉴定了8个玉米的PRMT蛋白序列,通过与两种模式植物(拟南芥和水稻)的全部PRMT蛋白序列的同源比对和系统发生关系分析,确定玉米PRMT蛋白主要分布在3个不同的亚家族中。运用生物信息学的方法和软件预测和分析了全部玉米PRMT蛋白氨基酸序列的理化性质、信号肽、跨膜结构域、疏水性和亲水性,以及蛋白质二级及三级结构等重要参数。这些数据对后续鉴定玉米PRMT蛋白的功能具有重要的意义。

关键词: 玉米, 组蛋白甲基化, PRMT结构域

Abstract: Protein arginine methyltransferases, PRMTs, are one of important epigenetic regulating factors,which are involved in modifying histone arginine methylation, changing chromatin structure and regulating gene expression in eukaryotic genome. In this study, a complete set of 8 PRMT proteins were identified in maize genome. By contrasting with the PRMT protein sequences in Arabidopsis and rice, all 8 ZmPRMT proteins were categorized into three classes based on phylogeny in maize. By using bioinformatics methods and softwares, physical and chemical of amino acid sequence of all maize PRMT proteins were analyzed, and their signal peptide, transmembrane domain, hydrophobic and hydrophilic structure, protein secondary and tertiary structure were also determined in this study. These results revealed a comprehensive overview of the maize PRMT protein family and provided a great significance on subsequent functional research of PRMT proteins in maize.

Key words: maize, histone methylation, PRMT domain