关键词: 玉米, 玉米灰斑病, 混池测序, 候选基因

Abstract: Gray leaf spot (GLS) caused by Cercospora zeine and Cercospora zeae-maydis is one of the crucial fungal foliar diseases throughout the world. In this study, we constructed one population of BC1F1 with GLS-resistance inbred line Suwan1 and GLS-susceptible inbred line HM01, which was regarded as recurrent parent. After evaluation of GLS resistance for the BC1F1 population in natural condition, one resistant and one susceptible DNA bulks were prepared, both of which included 30 extreme individuals. With the help of high-throughput sequencing technology, quality control and data analysis were utilized to detect genomic variant information in the two bulks. As a result, totally 29 resistant quantitative trait loci (QTL) containing 2 768 genes according to MaizeGDB website were identified via the statistical test of sequencing depth differences of the two bulks. In addition, 2 768 genes were projected onto Oryza sativa and Arabidopsis thaliana genome on the Phytozome website. Finally, three genes located at chromosome 1, 5 and 10 were selected as candidate genes of GLS resistance.

Key words: maize, gray leaf spot in maize, bulk sequencing, candidate gene