壳寡糖缓解娃娃菜幼苗盐胁迫的生理及光合响应

doi:10.19586/j.2095-2341.2025.0066

生物技术进展 ›› 2025, Vol. 15 ›› Issue (4): 675-682.DOI: 10.19586/j.2095-2341.2025.0066

• 研究论文 • 上一篇

壳寡糖缓解娃娃菜幼苗盐胁迫的生理及光合响应

鄂利锋¹(), 李亚妮¹, 谢钰², 权建华¹, 陈修斌¹, 华军³, 赵雪梅⁴, 赵文勤⁵

^1.河西学院农业与生态工程学院，甘肃张掖 734000
^2.高台县良种繁育推广中心，甘肃高台 734300
^3.张掖市经济作物技术推广站，甘肃张掖 734000
^4.甘州区农业技术推广中心，甘肃甘州 734000
^5.甘肃华美农业科技有限公司，甘肃张掖 734000

收稿日期:2025-05-26 接受日期:2025-06-13 出版日期:2025-07-25 发布日期:2025-09-08
作者简介:鄂利锋 E-mail: hxxyelf@163.com
基金资助:
甘肃省科技计划(技术创新引导计划)项目(24CXNG006);甘肃省农业农村厅农业科技支撑项目(KJZC-2024-27)

Physiological and Photosynthetic Responses of Brassica pekinensis Seedlings to Salt Stress Alleviation by Chitosan Oligosaccharide

Lifeng E¹(), Yani LI¹, Yu XIE², Jianhua QUAN¹, Xiubin CHEN¹, Jun HUA³, Xuemei ZHAO⁴, Wenqin ZHAO⁵

^1.College of Agriculture and Ecological Engineering，Hexi University，Gansu Zhangye 734000，China
^2.Gaotai County Elite Seed Breeding and Extension Center，Gansu Gaotai 734300，China
^3.Zhangye Economic Crops Technology Extension Station，Gansu Zhangye 734000，China
^4.Ganzhou District Agricultural Technology Extension Center，Gansu Ganzhou 734000，China
^5.Gansu Huamei Agricultural Technology Co. ，Ltd. ，Gansu Zhangye 734000，China

Received:2025-05-26 Accepted:2025-06-13 Online:2025-07-25 Published:2025-09-08

摘要/Abstract

摘要：

为探究壳寡糖（chitooligosaccharides，COS）对盐胁迫下娃娃菜幼苗生理特性的调控作用，以“耐寒金皇后”娃娃菜为材料，将0（CK）、50、100、150、200 mg·L^-1 COS喷施叶面4 d后，用150 mmol·L^-1 NaCl溶液对娃娃菜幼苗进行胁迫处理。结果发现，100 mg·L^-1 COS处理显著提高了NaCl胁迫下娃娃菜幼苗的株高和单株干重，增幅分别达14.44%和88.06%，同时显著提升抗氧化酶——过氧化物酶（peroxidase，POD）和过氧化氢酶（catalase，CAT）的活性，增幅分别为16.41%和33.77%，而丙二醛（malondialdehyde，MDA）含量显著下降；叶片净光合速率（Pn）、蒸腾速率（Tr）及气孔导度（Gs）显著提升，分别提高了28.26%、41.67%和15.90%，而胞间CO?浓度（Ci）显著降低，脯氨酸含量显著增加。结果表明，100 mg·L^-1 COS可通过增强抗氧化防御能力和光合效能，有效缓解盐胁迫对娃娃菜幼苗的伤害，为高原夏菜抗逆栽培提供了理论依据和技术支撑。

关键词: 壳寡糖, 娃娃菜, 盐胁迫, 生理特性, 光合参数

Abstract:

To investigate the regulatory effects of chitooligosaccharides （COS） on physiological characteristics of Brassica pekinensis seedlings under salt stress， the cultivar "Cold-tolerant Golden Empress" was used. Seedlings were foliar sprayed with 0 （CK）， 50， 100， 150， or 200 mg·L^-1 COS for 4 days and then subjected to salt stress with 150 mmol·L^-1 NaCl. The results showed that the 100 mg·L^-1 COS treatment significantly increased plant height and dry weight per plant under NaCl stress， increasing by 14.44% and 88.06%， respectively. It also significantly enhanced the activities of antioxidant enzymes peroxidase （POD） and catalase （CAT） by 16.41% and 33.77%， respectively， while significantly reducing malondialdehyde （MDA） content. Furthermore， the net photosynthetic rate （Pn）， transpiration rate （Tr）， and stomatal conductance （Gs） of the leaves were significantly increased by 28.26%， 41.67%， and 15.90%， respectively， whereas intercellular CO₂concentration （Ci） was significantly reduced. Proline content also increased significantly. These results indicated that 100 mg·L^-1 COS effectively alleviates salt stress-induced damage in Brassica pekinensis seedlings by enhancing antioxidant defense and photosynthetic efficiency， providing a theoretical basis and technical support for stress-resistant cultivation of highland summer vegetables.

Key words: chitooligosaccharide, Brassica pekinensis, salt stress, physiological characteristics, photosynthetic parameters

中图分类号:

Q949.748.3

鄂利锋, 李亚妮, 谢钰, 权建华, 陈修斌, 华军, 赵雪梅, 赵文勤. 壳寡糖缓解娃娃菜幼苗盐胁迫的生理及光合响应[J]. 生物技术进展, 2025, 15(4): 675-682.

Lifeng E, Yani LI, Yu XIE, Jianhua QUAN, Xiubin CHEN, Jun HUA, Xuemei ZHAO, Wenqin ZHAO. Physiological and Photosynthetic Responses of Brassica pekinensis Seedlings to Salt Stress Alleviation by Chitosan Oligosaccharide[J]. Current Biotechnology, 2025, 15(4): 675-682.

图/表 8

图1 COS诱导对NaCl胁迫下娃娃菜幼苗单株干重的影响注：不同小写字母表示处理之间差异在P<0.05水平上有统计学意义。

Fig. 1 Effects of COS induction on dry weight per plant of Brassica pekinensis seedlings under NaCl stress

图2 COS诱导对NaCl胁迫下娃娃菜幼苗株高的影响注：不同小写字母表示处理之间差异在P<0.05水平上有统计学意义。

Fig. 2 Effects of COS induction on plant height of Brassica pekinensis seedlings under NaCl stress

表1 COS诱导对NaCl胁迫下娃娃菜幼苗抗氧化酶活性的影响

Table 1 Effects of COS induction on the activities of antioxidant enzymes in Brassica pekinensis seedlings under NaCl stress

处理浓度/(mg·L^-1)	MDA含量/(nmol·g^-1)	POD活性/(U·g^-1 FW)	CAT活性/(U·g^-1 FW)
CK	63.13 a	611.27 e	355.27 e
50	44.24 c	681.27 b	431.57 b
100	31.53 e	711.56 a	475.23 a
150	44.63 b	677.47 c	425.36 c
200	40.34 d	657.27 d	405.49 d

图3 COS诱导对NaCl胁迫下娃娃菜幼苗净光合速率的影响注：不同小写字母表示处理之间差异在P<0.05水平有统计学意义。

Fig. 3 Effects of COS induction on net photosynthetic rate of Brassica pekinensis seedlings under NaCl stress

图4 COS诱导对NaCl胁迫下娃娃菜幼苗气孔导度的影响注：不同小写字母表示处理之间差异在P<0.05水平有统计学意义。

Fig. 4 Effects of COS induction on stomatal conductance of Brassica pekinensis seedlings under NaCl stress

图5 COS诱导对NaCl胁迫下娃娃菜幼苗蒸腾速率的影响注：不同小写字母表示处理之间差异在P<0.05水平有统计学意义。

Fig. 5 Effects of COS induction on transpiration rate of Brassica pekinensis seedlings under NaCl stress

图6 COS诱导对NaCl胁迫下娃娃菜幼苗胞间CO2浓度的影响注：不同小写字母表示处理之间差异在P<0.05水平有统计学意义。

Fig. 6 Effect of COS induction on intercellular CO2 concentration of Brassica pekinensis seedlings under NaCl stress

图7 COS诱导对NaCl胁迫下娃娃菜幼苗脯氨酸的影响注：不同小写字母表示处理之间差异在P<0.05水平有统计学意义。

Fig. 7 Effects of COS induction on proline in Brassicapekinensis seedlings under NaCl stress

参考文献 31

[1]	魏周秀, 张文斌, 王勤礼, 等. 张掖市钢架大棚秋茬娃娃菜品种比较试验[J]. 农业科技与信息, 2022, 19(8): 13-15.
	WEI Z X, ZHANG W B, WANG Q L, et al.. Comparative experiment on varieties of baby cabbage in autumn in steel frame greenhouse in Zhangye city[J]. Agric. Sci. Technol. Inf., 2022, 19(8): 13-15.
[2]	杨彬, 陈修斌, 钟玉风, 等. 水氮互作对黑河中游娃娃菜光合特性与水氮利用的影响[J]. 土壤通报, 2017, 48(1):162-168.
	YANG B, CHEN X B, ZHONG Y F, et al.. Effects of water and nitrogen interaction on photosynthetic characteristics and water-nitrogen utilization of baby cabbage in middle reaches of Hei River[J]. Chin. J. Soil Sci., 2017, 48(1): 162-168.
[3]	湛长菊. 娃娃菜的特征特性及高产栽培技术[J]. 现代农业科技, 2008(19): 63-63+65.
	ZHAN C J. Characteristics and high-yield cultivation techniques of baby cabbage[J]. Mod. Agric. Sci. Technol., 2008(19): 63-63.
[4]	MAO W, KANG S, WAN Y, et al.. Yellow River sediment as a soil amendment for amelioration of saline land in the Yellow River Delta[J]. Land Degrad. Dev., 2016, 27(6): 1595-1602.
[5]	王耀琳. 甘肃景泰盐碱地枸杞林生态系统效益[D]. 兰州: 兰州大学, 2015.
[6]	袁广祥, 陈德文, 朱井生, 等. 河西走廊水资源开发利用诱发土壤盐渍化的风险评价[J]. 中国农村水利水电, 2021(10):162-167.
	YUAN G X, CHEN D W, ZHU J S, et al.. Risk assessment of soil salinization induced by water resources development and utilization in Hexi Corridor[J]. China Rural. Water Hydropower, 2021(10): 162-167.
[7]	汪卫国, 宋西德. 甘肃河西走廊盐碱地改造利用技术探讨[J]. 陕西林业科技, 2006(4): 6-9.
	WANG W G, SONG X D. Discussion on the improvement and utilization of saline-alkali land in Hexi Corridor of Gansu[J]. Shaanxi For. Sci. Technol., 2006(4): 6-9.
[8]	邓拓, 张金龙, 冯纪年, 等. 壳聚糖对低温胁迫下核桃抗冻反应的激发作用[J]. 西北林学院学报, 2016, 31(1): 60-64.
	DENG T, ZHANG J L, FENG J N, et al.. Elicitation effect of chitosan on cold tolerance of walnut tree under cold stress[J]. J. Northwest For. Univ., 2016, 31(1): 60-64.
[9]	YADAV S, IRFAN M, AHMAD A, et al.. Causes of salinity and plant manifestations to salt stress: a review[J]. J. Environ. Biol., 2011, 32(5): 667-685.
[10]	BARKLA B J, CASTELLANOS-CERVANTES T, DE LEÓN J L D, et al.. Elucidation of salt stress defense and tolerance mechanisms of crop plants using proteomics: current achievements and perspectives[J]. Proteomics, 2013, 13(12-13): 1885-1900.
[11]	魏嘉, 蔡勤安, 李源, 等. 植物对盐碱胁迫响应机制的研究进展[J]. 山东农业科学, 2022, 54(4): 156-164.
	WEI J, CAI Q A, LI Y, et al.. Research progress on response M echanism of the plant to saline-a lkali stress[J]. Shandong Agric. Sci., 2022, 54(4): 156-164.
[12]	杨发斌, 杨晓刚, 薛梦迪, 等. 18个大白菜品种耐盐性筛选[J]. 山东农业科学, 2017, 49(3): 31-36.
	YANG F B, YANG X G, XUE M D, et al.. Screening of salt tolerantce of eighteen Chinese cabbage varieties[J]. Shandong Agric. Sci., 2017, 49(3): 31-36.
[13]	邱念伟, 刘倩, 王凤德, 等. 大白菜不同发育阶段耐盐性的长期观察[J]. 植物生理学报, 2015, 51(10): 1597-1603.
	QIU N W, LIU Q, WANG F D, et al.. Long-term observation on salt tolerance of Chinese cabbage at different development stages[J]. Plant Physiol. J., 2015, 51(10): 1597-1603.
[14]	郭亚宁, 艾静. 盐胁迫对油白菜生长及其生理指标的影响[J]. 榆林学院学报, 2023, 33(2): 38-41.
	GUO Y N, AI J. Effects of salt stress on morphological and physiological index of Chinese cabbage[J]. J. Yulin Univ., 2023, 33(2): 38-41.
[15]	祁玉洁. 探索盐碱地治理的"甘肃良方"[N]. 甘肃经济日报,2023-12-29(001).
[16]	陆建玲, 孙达峰, 张超, 等. 壳寡糖对辣椒种子萌发及幼苗抗氧化酶活性影响研究[J]. 中国野生植物资源, 2012, 31(2):12-16.
	LU J L, SUN D F, ZHANG C, et al.. Effects of chitosan oligosaccharide on pepper's seed germination and seedling antioxidant enzyme activity[J]. Chin. Wild Plant Resour., 2012, 31(2): 12-16.
[17]	孙凤岭, 陈昆, 姜涛. 盐胁迫对西瓜幼苗生物量及生理特性的影响[J]. 现代农业科技, 2023(7): 63-65.
	SUN F L, CHEN K, JIANG T. Effects of salt stress on biomass and physiological characteristics of watermelon seedlings[J]. Mod. Agric. Sci. Technol., 2023(7): 63-65.
[18]	王嘉欣, 高艳明, 李建设, 等. 壳寡糖对硬水灌溉下黄瓜育苗效果的影响[J]. 蔬菜, 2022(3): 13-20.
	WANG J X, GAO Y M, LI J S, et al.. Effects of chitosan oligosaccharide on cucumber seedling growth under hard water irrigation[J]. Vegetables, 2022(3): 13-20.
[19]	刘强, 王庆成, 王占武, 等. 外源壳寡糖对苍耳盐害的缓解作用[J]. 东北林业大学学报, 2014, 42(6): 73-75.
	LIU Q, WANG Q C, WANG Z W, et al.. Alleviation of salt stress injury in Xanthium sibiricum by exogenous oligochitosan[J]. J. Northeast. For. Univ., 2014, 42(6): 73-75.
[20]	朱迎春, 孙德玺, 刘君璞, 等. 壳寡糖对NaCl胁迫下耐盐性不同西瓜幼苗生理特性的影响[J]. 果树学报, 2020, 37(6):866-874.
	ZHU Y C, SUN D X, LIU J P, et al.. Effects of chitosan oligosaccharides on watermelon seedlings with different salt tolerance under NaCl stress[J]. J. Fruit Sci., 2020, 37(6): 866-874.
[21]	曾雨琴. 壳寡糖对海盐胁迫下白菜植株生理生化指标的影响[D]. 南京: 南京农业大学, 2020.
[22]	丁振中, 曾哲灵, 龚劲松, 等. NaCl胁迫下壳寡糖对油菜苗生长的影响[J]. 生物化工, 2018(3): 29-33.
	DING Z Z, ZENG Z L, GONG J S, et al.. Effects of chitooligosaccharides on the growth of rape seedlings stressed with NaCl[J]. Biol. Chem. Eng., 2018(3): 29-33.
[23]	任雪梅, 王文特, 田洪芸, 等. 比色法测定鸭油中的丙二醛[J]. 山东农业科学, 2014, 46(1): 117-119.
	REN X M, WANG W T, TIAN H Y, et al.. Determination of malondialdehyde content in duck oil by colorimetry[J]. Shandong Agric. Sci., 2014, 46(1): 117-119.
[24]	杨兰芳, 曾巧, 李海波, 等. 紫外分光光度法测定土壤过氧化氢酶活性[J]. 土壤通报, 2011, 42(1): 207-210.
	YANG L F, ZENG Q, LI H B, et al.. Measurement of catalase activity in soil by ultraviolet spectrophotometry[J]. Chin. J. Soil Sci., 2011, 42(1): 207-210.
[25]	李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000.
[26]	韩志平, 郭世荣, 冯吉庆, 等. 盐胁迫对西瓜幼苗生长、叶片光合色素和脯氨酸含量的影响[J]. 南京农业大学学报,2008, 31(2): 32-36.
	HAN Z P, GUO S R, FENG J Q, et al.. Effect of salinity on plant growth, photosynthetic pigments and proline content in leaves of watermelon seedlings[J]. J. Nanjing Agric. Univ., 2008, 31(2): 32-36.
[27]	刘建新, 欧晓彬, 王金成. 外源H₂O₂对盐碱混合胁迫下裸燕麦幼苗生长和抗性生理的影响[J]. 植物研究, 2019, 39(2):181-191.
	LIU J X, OU X B, WANG J C. Effects of exogenous hydrogen peroxide on growth and resistance physiology of naked oat seedlings under saline-alkali mixed stress[J]. Bull. Bot. Res., 2019, 39(2): 181-191.
[28]	赵肖琼, 梁泰帅, 张恒慧. 壳寡糖对NaCl胁迫下盆栽小白菜生长和品质的影响[J]. 北方园艺, 2020(15): 32-37.
	ZHAO X Q, LIANG T S, ZHANG H H. Effects of chitooligosaccharide on growth and quality of Brassica chinensis L. seedlings under NaCl stress[J]. North. Hortic., 2020(15): 32-37.
[29]	张帅磊, 罗石磊, 张文渊, 等. 2,4-表油菜素内酯对盐胁迫下黄瓜幼苗叶片光合特性的影响[J]. 西北植物学报, 2022, 42(2): 272-279.
	ZHANG S L, LUO S L, ZHANG W Y, et al.. Effects of 2, 4-epibrassinolide on photosynthetic characteristics of cucumber seedlings under salt stress[J]. Acta Bot. Boreali Occidentalia Sin., 2022, 42(2): 272-279.
[30]	杨顺. 壳寡糖对盐胁迫下甘蓝生长生理及产量品质的影响[D]. 杨凌: 西北农林科技大学,2023.
[31]	石欣隆, 杨月琴, 薛娴, 等. 壳寡糖对干旱胁迫下'凤丹'幼苗生长及生理特性的影响[J]. 南京林业大学学报(自然科学版), 2021, 45(2): 120-126.
	SHI X L, YANG Y Q, XUE X, et al.. Effects of chitosan oligosaccharide on growth and physiological characteristics of 'Fengdan' seedlings under drought stress[J]. J. Nanjing Forestry Univ. (Nat. Sci. Edit.), 2021, 45(2): 120-126.

壳寡糖缓解娃娃菜幼苗盐胁迫的生理及光合响应

Physiological and Photosynthetic Responses of Brassica pekinensis Seedlings to Salt Stress Alleviation by Chitosan Oligosaccharide

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 8

参考文献 31

相关文章 7

编辑推荐

Metrics

本文评价

[1]	刘卓颖, 周晓今, 黄燕丽, 逄森. 玉米盐胁迫和MeJA处理下的转录组联合分析[J]. 生物技术进展, 2025, 15(2): 263-275.
[2]	张奇雨, 吕晓桂, 包锦. 冷等离子体处理对盐胁迫下燕麦种子萌发及幼苗生长的影响[J]. 生物技术进展, 2024, 14(3): 413-421.
[3]	张香香,滕炎桐,陈涛. AtEXD参与植物盐胁迫的作用研究[J]. 生物技术进展, 2021, 11(1): 61-68.
[4]	原旭冰,刘洪涛,杜昱光. 壳寡糖的制备及其在医学和农业生产中的应用[J]. 生物技术进展, 2018, 8(6): 461-468.
[5]	陈新兵,黄荣峰,王娟. 水稻矮杆突变体D814基因图位克隆与功能分析[J]. 生物技术进展, 2017, 7(6): 608-617.
[6]	葸玉琴,马春林,曹云涛,孔维宝,杨红. 自养和混养小球藻对NaCl胁迫的生理响应[J]. 生物技术进展, 2013, 3(3): 223-228.
[7]	陈文俊,田孟良,张军杰,黄玉碧,李建生,杨华,付忠军,郑阳. 玉米叶片持绿性研究进展与对策[J]. 生物技术进展, 2011, 1(6): 409-412.