关键词: 肝纤维化, 单细胞测序, 空间转录组, 纤维化机制, 细胞相互作用

Abstract:

Hepatic fibrosis poses a significant threat to human health. Single-cell transcriptome sequencing technology offers a novel approach to elucidate its complex pathological mechanisms. Traditional research methods are limited in identifying the different cell subpopulations and their gene expression changes in hepatic fibrosis， making it difficult to fully understand the disease mechanisms. This paper summarized the research progress of single-cell transcriptome sequencing technology in identifying various cell subtypes during the process of hepatic fibrosis. Single-cell RNA sequencing technology can precisely analyze the gene expression and heterogeneity of different cell types， revealing the dynamic changes in cell subpopulations and their gene expression during hepatic fibrosis. This， in turn， helped in understanding the functions， interactions， and contributions of various cell subtypes to the progression of the disease. The paper further discussed the significance and prospects of this technology in hepatic fibrosis research. By using this technology， key genes and signaling pathways related to fibrosis can be identified， providing a theoretical basis for early diagnosis， the discovery of therapeutic targets， and the development of new treatments. Additionally， with the integration of spatial transcriptome sequencing technology， researchers can observe the spatial distribution of cells within tissues， further enhancing the understanding of the microenvironment of hepatic fibrosis. This technology aids in deepening the understanding of the pathological mechanisms of hepatic fibrosis and offers innovative ideas for discovering new therapeutic targets and developing strategies for early diagnosis and treatment.

Key words: liver fibrosis, single-cell sequencing, spatial transcriptomics, fibrosis mechanisms, cell-cell interactions