合成生物学在治疗代谢性疾病中的研究进展

doi:10.19586/j.2095-2341.2022.0196

生物技术进展 ›› 2023, Vol. 13 ›› Issue (3): 383-389.DOI: 10.19586/j.2095-2341.2022.0196

合成生物学在治疗代谢性疾病中的研究进展

白亮¹^,²(), 黄鹤¹, 王苹²()

^1.天津大学化工学院，天津 300380
^2.天津现代创新中药科技有限公司，天津 300392

收稿日期:2022-11-21 接受日期:2023-03-10 出版日期:2023-05-25 发布日期:2023-06-12
通讯作者: 王苹
作者简介:白亮 E-mail：bailiang20101@163.com；
基金资助:
国家重点研发计划项目(2019YFA09005600)

Advances in Synthetic Biology on the Treatment of Metabolic Diseases

Liang BAI¹^,²(), He HUANG¹, Ping WANG²()

^1.School of Chemical Engineering and Technology，Tianjin University，Tianjin 300380，China
^2.Modern Traditional Chinese Medicine Innovation Center Co. ，Ltd，Tianjin 300392，China

Received:2022-11-21 Accepted:2023-03-10 Online:2023-05-25 Published:2023-06-12
Contact: Ping WANG

摘要/Abstract

摘要：

代谢性疾病（metabolic disease，MD）一直以来是健康领域面临的重大挑战，其发生发展与宿主的代谢和免疫存在密切联系。近年来基于肠道菌群的治疗策略层出不穷，特别是利用合成生物学改造的微生物，对于代谢性疾病的预防和治疗展现出良好的应用潜力。通过对微生物基因路线和代谢途径进行合理设计和改造，可以实现目标物质的稳定输出和精准投递，进而治疗疾病。着重综述了合成生物学在代谢性疾病预防和治疗中的研究进展，以期为改善和治疗代谢性疾病提供新的思路和方法。

关键词: 代谢性疾病, 肥胖, 合成生物学, 工程菌, 肠道菌群

Abstract:

Metabolic diseases （MD） has been a major challenge in health care and its occurrence and development are closely related to the metabolism and immunity. In recent years， treatment strategies based on gut microbiota have incompleted， especially the microorganisms modified by synthetic biology， which show good potential application for the prevention and treatment of MD. To cure diseases， the design and transformation of microbial gene routes and metabolic pathways can achieve stable output and accurate of target substances. This review focused on the research progress of synthetic biology in the prevention and treatment of MD， with a view to providing new ideas and methods for improving and treating MD.

Key words: metabolic disease, obesity, synthetic biology, engineering bacteria, gut mircobiota

中图分类号:

R598

白亮, 黄鹤, 王苹. 合成生物学在治疗代谢性疾病中的研究进展[J]. 生物技术进展, 2023, 13(3): 383-389.

Liang BAI, He HUANG, Ping WANG. Advances in Synthetic Biology on the Treatment of Metabolic Diseases[J]. Current Biotechnology, 2023, 13(3): 383-389.

图/表 1

参考文献 51

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适应症	靶标物质	作用机制	参考文献
酒精性脂肪肝	IL-22	上调肠道Reg3g表达，减少细菌移位至肝脏，缓解肝损伤	[18]
高血氨症	苯丙氨酸	构建Phe代谢途径，降低血氨浓度	[20-22]
肥胖	GLP-1	促进胰岛素的分泌，改善血脂代谢，调节肠道菌群组成	[24,30]
	丁酸	改善物质代谢，调节肠道菌群组成	[28,32]
	泛连接蛋白	与Gβγ亚基结合后，通过β3AR刺激而被激活，调节细胞产热	[33]
糖尿病	筛选抑制剂	筛选β细胞分化抑制剂	[38]
	BSH和IL-10	响应乳糖浓度信号，调节肠道菌群组成	[39]
	阿那白滞素	改善物质代谢，抵抗炎症	[40-41]
乳糖不耐受	乳糖	响应乳糖浓度信号，调节肠道菌群组成	[42]
庞贝病	α-葡萄糖苷酶	构建编码α-葡萄糖苷酶，降解糖原浓度	[44]
粘多糖症	蛋白聚糖	构建编码人α-N-乙酰氨基葡萄糖苷酶，降解蛋白聚糖浓度	[51]

适应症	靶标物质	作用机制	参考文献
酒精性脂肪肝	IL-22	上调肠道Reg3g表达，减少细菌移位至肝脏，缓解肝损伤	[18]
高血氨症	苯丙氨酸	构建Phe代谢途径，降低血氨浓度	[20-22]
肥胖	GLP-1	促进胰岛素的分泌，改善血脂代谢，调节肠道菌群组成	[24,30]
	丁酸	改善物质代谢，调节肠道菌群组成	[28,32]
	泛连接蛋白	与Gβγ亚基结合后，通过β3AR刺激而被激活，调节细胞产热	[33]
糖尿病	筛选抑制剂	筛选β细胞分化抑制剂	[38]
	BSH和IL-10	响应乳糖浓度信号，调节肠道菌群组成	[39]
	阿那白滞素	改善物质代谢，抵抗炎症	[40-41]
乳糖不耐受	乳糖	响应乳糖浓度信号，调节肠道菌群组成	[42]
庞贝病	α-葡萄糖苷酶	构建编码α-葡萄糖苷酶，降解糖原浓度	[44]
粘多糖症	蛋白聚糖	构建编码人α-N-乙酰氨基葡萄糖苷酶，降解蛋白聚糖浓度	[51]

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合成生物学在治疗代谢性疾病中的研究进展

Advances in Synthetic Biology on the Treatment of Metabolic Diseases

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