甘露糖赤藓糖醇脂生产及应用研究进展

doi:10.19586/j.2095-2341.2022.0131

生物技术进展 ›› 2023, Vol. 13 ›› Issue (2): 210-219.DOI: 10.19586/j.2095-2341.2022.0131

• 进展评述 • 上一篇

甘露糖赤藓糖醇脂生产及应用研究进展

邱思元(), 徐晶雪(), 段育阳, 赵金玉, 赵文婧, 张莉欣, 任国领

大庆师范学院生物工程学院，黑龙江大庆 163712

收稿日期:2022-07-12 接受日期:2022-11-17 出版日期:2023-03-25 发布日期:2023-04-07
通讯作者: 徐晶雪
作者简介:邱思元 E-mail：1178735617@qq.com；
基金资助:
黑龙江省联合引导基金项目(LH2020C070);2022年黑龙江省大学生创新创业训练计划项目(S202210235030)

Research Progress on Production and Application of Mannosylerythritol Lipids

Siyuan QIU(), Jingxue XU(), Yuyang DUAN, Jinyu ZHAO, Wenjing ZHAO, Lixin ZHANG, Guoling REN

College of Bioengineering，Daqing Normal University，Heilongjiang Daqing 163712，China

Received:2022-07-12 Accepted:2022-11-17 Online:2023-03-25 Published:2023-04-07
Contact: Jingxue XU

摘要/Abstract

摘要：

甘露糖赤藓糖醇脂（mannosylerythritol lipids, MELs）是一种生物表面活性剂，除具有可降解、毒性低、生物兼容性好等优点，还因其特有的代谢、合成途径与结构特性，而具有基因转染、广谱抗菌、皮肤修复等多种功能。MELs在医疗、日化、食品、农业、生态修复等各领域应用前景巨大，被公认为是现今最有潜力的生物表面活性剂。然而，不同种属所生产的MELs之间结构差异性大且生产方式较落后，合成与作用机制尚不清晰，因而无法实现规模商业化生产。从结构特性、生产纯化、应用途径等方面重点阐述了MELs相关研究进展，以期阐明其结构与功能的多样性，为实现靶向MELs的定制生产，降低生产成本，加快实现其规模化应用提供参考。

关键词: 甘露糖赤藓糖醇脂, 生物表面活性剂, 微生物

Abstract:

As　a type of biosurfactant， mannosylerythritol lipids （MELs） have merits with biodegradability， low toxicity and good biocompatibility. Due to its distinct structural， metabolic， and synthetic features， it also performs a wide range of other tasks， including skin restoration， gene transfection， and broad-spectrum antibacterial activity. MELs has a promising future in a number of industries， including medicine， daily chemicals， food， agriculture， and ecological restoration， which is widely recognized as one of the most promising biosurfactants. However， because of the great structural heterogeneity， backward manufacturing style of MELs generated by various species， hazy synthesis and uncertain mechanism of action， it is hard to carry out large-scale commercial production. The paper aimed to clarify the diversity of its structure and function， so as to provide reference for the realization of customized production targeting MELs. By focusing on the aspects of structural characteristics， production purification， application path and so on， the paper was hoped to help reduce the production cost and accelerate the realization of its large-scale application.

Key words: mannosylerythritol lipids, bio-surfactants, microorganism

中图分类号:

Q549

邱思元, 徐晶雪, 段育阳, 赵金玉, 赵文婧, 张莉欣, 任国领. 甘露糖赤藓糖醇脂生产及应用研究进展[J]. 生物技术进展, 2023, 13(2): 210-219.

Siyuan QIU, Jingxue XU, Yuyang DUAN, Jinyu ZHAO, Wenjing ZHAO, Lixin ZHANG, Guoling REN. Research Progress on Production and Application of Mannosylerythritol Lipids[J]. Current Biotechnology, 2023, 13(2): 210-219.

图/表 4

图1 MELs的化学式注：MEL-A：R1=R2=Ac；MEL-B：R1=Ac，R2=H；MEL-C：R1=H，R2=Ac。n=6~10

Fig. 1 Chemical formula of MELs

图2 甘露糖基赤藓糖醇脂的合成途径注：meso-Erythritol—赤藓醇；Emt1—糖基转移酶；Mac1、Mac2—酰基转移酶；Mat1—乙酰基转移酶；n＝6或8。ME—甘露糖基赤藓糖醇；MEL-D—脱乙酰化甘露糖基赤藓糖醇脂质；MEL-C—4′-乙酰化甘露糖基赤藓糖醇脂质[9]。

Fig. 2 Lipid synthesis pathway of mannosyl erythritol

表1 常见的MELs生物来源及所产类型

Table 1 Biological sources and types of common MELs

菌株种类	主产类型	参考文献
P. antarctica	MEL-A	[12]
P. churashimeensis	MEL-A	[12]
P. fusiformata	MEL-A	[12]
P. parantarctica	MEL-A	[9]
P. aphids	MEL-A	[12]
P. rugulosa	MEL-A	[9]
南极假单胞杆菌	MEL-A	[8]
P. crassa	MEL-A,-B	[13]
P. tsukubaensis	MEL-B	[13]
P. hubeiensis	MEL-C	[13]
P. graminicola	MEL-C	[12]
P. siamensis	MEL-C	[12]
P. shanxiensis	MEL-C	[15]
湖北锥菌SY62	MEL-C	[8]
P. antractia PYCC 5048	MELs	[15]
P. aphidis PYCC 5535	MELs	[15]
P. antarctious	MEL-C,-D	[8]
Ustilago scitaminee	MEL-A,-B	[8]
Ustilago maydis	MEL-A,-B,-C	[12]
Uredo cynodontis	MEL-C	[12]
P. siamensis CBS 9906	MEL-C	[14]

图3 MEL生产的时间过程和干燥细胞重量[19]注：Batch—分批补料培养；Fed-(a)—增添大豆油；Fed-(b)—增添大豆油与酵母提取物；Fed-(c)—增添大豆油、酵母提取物和葡萄糖。

Fig. 3 Time course of MEL production and dried cell weight [19]

参考文献 40

1	LEU J Y, YEE J, TU C S, et al.. Microstructure and molecular vibration of mannosylerythritol lipids from Pseudozyma yeast strains[J/OL]. Chem. Physics Lipids, 2020, 232: 104969[2022-11-17]. .
2	TOMOTAKE M, YUKI O, MASAKO T, et al.. Isolation of Pseudozyma churashimaensis sp. nov.,a novel ustilaginomycetous yeast species as a producer of glycolipid biosurfactants, mannosylerythritol lipids[J]. J. Biosci. Bioengin., 2011, 112: 137-144.
3	AZUSA S, YU U, HIDEAKI K, et al.. Identification of the gene PtMAT1 encoding acetyltransferase from the diastereomer type of mannosylerythritol lipid-B producer Pseudozyma tsukubaensis [J]. J. Biosci. Bioengin., 2018, 126: 676-681.
4	KONDO T, YASUI C, BANNO T, et al.. Self‐assembling properties and recovery effects on damaged skin cells of chemically synthesized mannosylerythritol lipids[J/OL]. ChemBioChem, 2022, 23(2): e202100631[2021-11-16]. .
5	范琳琳.甘露糖赤藓糖醇脂的生物合成及性质与应用研究[D].杭州:浙江大学,2015.
6	FABIENNE B, THORSTEN S, UWE L, et al.. Engineering ustilago maydis for production of tailor-made mannosylerythritol lipids[J/OL]. Metabolic Engin. Commun., 2021, 12: e00165[2022-11-17]. .
7	TOKUMA F, TAKASHI Y, TOMOHIRO L, et al.. The diastereomers of mannosylerythritol lipids have different interfacial properties and aqueous phase behavior, reflecting the erythritol configuration[J]. Carbohydr. Res., 2012, 351: 81-86.
8	CLOSES H, LINNE U, SCHERER M,et al.. Identification of a gene cluster for biosynthesis of mannosylerythritol lipids in the basidiomycetous fungus Ustilago maydis Appl.[J]. Environ. Microbiol., 2006, 72: 5469-5477.
9	MASAAKI K, MOTOKI M. Selective production of deacetylated mannosylerythritol lipid, MEL-D, by acetyltransferase disruption mutant of Pseudozyma hubeiensis [J]. J. Biosci. Bioengin., 2018, 125: 105-110.
10	孟令一.一株产糖脂类表面活性剂菌株的筛选、纯化及其产物分析[D].长春:东北师范大学,2015.
11	段正巧. Pseudozyma aphidis CNm2012代谢产物甘露糖赤藓糖醇脂(MELs)的抗菌活性检测[D].重庆:西南大学,2015.
12	TOMOTAKE M, MASAAKI K, TOKUMA F, et al.. Microbial conversion of glycerol into glycolipid biosurfactants, mannosylerythritol lipids, by a basidiomycete yeast, Pseudozyma antarctica JCM 10317[J]. J Biosci. Bioengin., 2007(1):78-81.
13	CRISTIANO J D, LIDIANE M D, SILVANA A, et al.. A novel approach for the production and purification of mannosylerythritol lipids (MEL) by Pseudozyma tsukubaensis using cassava wastewater as substrate[J]. Separ. Purif. Technol., 2017,180(8):157-167.
14	SHEN L, ZHUB J, LUB J, et al.. Isolation and purification of biosurfactant mannosylerythritol lipids from fermentation broth with methanol/water/n-hexane[J]. Sep. Purif. Technol., 2019, 219: 1-8.
15	薛婷婷,付瑞敏,谷亚楠,等.一株产甘露糖赤藓糖醇脂菌株的筛选及其产物分析[J].微生物学通报,2016,43:1210-1217.
16	TOKUMA F, MAYO K, TOMOTAKE M, et al.. A basidiomycetous yeast, Pseudozyma crassa, produces novel diastereomers of conventional mannosylerythritol lipids as glycolipid biosurfactants[J]. Carbohydr. Res., 2008, 343: 2947-2955.
17	华兆哲,陈坚,朱文昌,等.新型生物表面活性剂甘露糖赤藓糖醇脂(MEL)的分离与表面性质研究[J].天然产物研究与开发,1999(3):11-16.
18	NASCIMENTO M F, BARREIROS R, OLIVEIRA A C, et al.. Moesziomyces spp. cultivation using cheese whey: new yeast extract-free media, β-galactosidase biosynthesis and mannosylerythritol lipids production[J]. Biomass Conver. Biorefinery, 2022, 6: 1-14.
19	SAIKA A, FUKUOKA T, KOIKE H.A putative transporter gene PtMMF1-deleted strain produces mono-acylated mannosylerythritol lipids in Pseudozyma tsukubaensis [J]. Appl. Microbiol. Biotechnol., 2020, 104: 10105-10117.
20	TRAN Q, RYUA G, CHOI J. Enhanced production of biosurfactants through genetic engineering of Pseudozyma sp. SY16[J].Korean J. Chem. Eng.,2022, 39(4): 997-1003.
21	MASAAKI K, TOMOTAKE M, TOKUMA F, et al.. Efficient production of mannosylerythritol lipids with high hydrophilicity by Pseudozyma hubeiensis KM-59[J]. Appl. Microbiol. Biotechnol., 2008, 78: 37-46.
22	RAU U, NGUYEN L A, ROEPER H, et al.. Fed-batch bioreactor production of mannosylerythritol lipids secreted by Pseudozyma aphidis [J]. Appl. Microbiol. Biotechnol., 2005, 68: 607-613.
23	YU G, WANG X, ZHANG C, et al.. Efficient production of mannosylerythritol lipids by a marine yeast Moesziomyces aphidis XM01 and their application as self-assembly nanomicelles[J]. Marine Life Sci. Technol., 2022, 4(3): 373-383.
24	FAN L, CHEN Q, YASHENG M, et al.. Stable vesicle self-assembled from phospholipid and mannosylerythritol lipid and its application in encapsulating anthocyanins[J/OL]. Food Chem., 2021, 344: 128649[2022-11-17]. .
25	KITAMOTO D, ISODA H, NAKAHARA T. Functions and potential applications of glycolipid biosurfactants-from energy-saving materials to gene delivery carriers[J]. J. Biosci. Bioengin., 2002, 94: 187-201.
26	INOH Y, KITAMOTO D, HIRASHIMA N, et al.. Biosurfactants of MEL-A increase gene transfection mediated by cationic liposomes[J]. Biochem. Biophys. Res. Commun., 2001, 289: 57-61.
27	HANAA A H, MOHD A A. Biosurfactant as a vehicle for targeted antitumor and anticancer drug delivery[J]. Green Sustain. Process Chem. Environ. Engin. Sci., 2022, 16: 676-618.
28	TOMOTAKE M, MASARU K, SHUHEI Y, et al.. Glycolipid biosurfactants, Mannosy lerythritol lipids, repair the damaged hair[J]. J. Oleo Sci., 2010, 61: 407-412.
29	SHUHEI Y, TOMOTAKE M, TOKUMA F, et al.. The moisturizing effects of glycolipid biosurfactants,mannosylerythritol lipids, on human skin[J]. J. Oleo. Sci., 2012, 216: 676-618.
30	JING C, GUO J, LI Z, et al.. Screening and research on skin barrier damage protective efficacy of different mannosylerythritol lipids[J/OL].Molecules,2022,27:4648[2022-11-17]. .
31	LIU X, ZHANG L, PANG X, et al.. Synergistic antibacterial effect and mechanism of high hydrostatic pressure and mannosylerythritol lipid-A on Listeria monocytogenes [J/OL]. Food Control., 2022, 135: 108797[2022-11-17]. .
32	SHU Q, WEI T, LIU X, et al.. The dough-strengthening and spore-sterilizing effects of mannosylerythritol lipid-A in frozen dough and its application in bread making [J/OL]. Food Chem., 2022, 369: 131011[2022-11-17]. .
33	TOKUMA F, SHIGENOBU Y, JUNICHI N, et al.. Application of yeast glycolipid biosurfactant, mannosylerythritol lipid, as agrospreaders[J]. J. Oleo Sci., 2015, 64: 689-695.
34	TAHIR Z, NAZIR M S, ASLAM A A, et al.. Active metabolites and biosurfactants for utilization in environmental remediation and eco-restoration of polluted soils[J]. Biosurfact. Bioremed. Pollut. Environ., 2021(7):31-51.
35	RAJASIMMAN M, SUGANYA A, MANIVANNAN P, et al.. Utilization of agroindustrial wastes with a high content of protein, carbohydrates, and fatty acid used for mass production of biosurfactant[J]. Chem. Environ. Engin. Sci., 2021, 6: 127-146.
36	PHILLIP J, ANNA T, VICTOR S, et al.. Effect of synthetic surfactants on the environment and the potential for substitution by biosurfactants[J]. Adv. Colloid Interface Sci., 2021, 288: 676-618.
37	BELHAJ A, ELRAIES F. The effect of surfactant concentration, salinity, temperature, and pH on surfactant adsorption for chemical enhanced oil recovery: a review[J]. Petrol Explor. Prod. Technol., 2020, 10: 125-137.
38	HANS-TOBIAS D, UWE L, XIULAN X, et al.. Elucdation of substrate specificities of decorating enzymes involved in mannosylerythritol lipid production by cross-species complementation[J]. Fungal Genet. Biol., 2019, 130: 91-97.
39	CRISTIANO J DE A， ANA L S C， PAULO E F， et al.. Mannosylerythritol lipids： production， downstream processing， and potential applications‍［J/OL］. Curr. Opin. Biotechnol.， 2022， 77：102769［2022-11-17］..
40	FUKUOKA T, YANAGIHARA T, IMURA T, et al.. The diastereomers of mannosylerythritol lipids have different interfacial properties and aqueous phase behavior, reflecting the erythritol configuration[J]. Carbohydr. Res., 2012, 351: 81-86.

[1]	郝捷, 季嫱, 李力群, 郑超, 吴娜, 吴晗, 李选文, 孙志康. 生物酶和微生物技术改善烟叶香气的研究进展[J]. 生物技术进展, 2022, 12(6): 817-824.
[2]	刘培敏, 罗金萍, 高权新. 水产养殖环境微生物研究进展[J]. 生物技术进展, 2022, 12(5): 690-695.
[3]	李力群, 孙志康, 郝捷, 季嫱, 李选文, 吴晗, 吴娜, 郑超, 杨婧. 果胶酶生产及工业应用进展[J]. 生物技术进展, 2022, 12(4): 549-558.
[4]	李伟, 王冲, 刘嗣嘉, 杨敏一, 张云平. 宏基因组学技术在痤疮研究中的应用进展[J]. 生物技术进展, 2021, 11(6): 694-699.
[5]	赵冬雪,刘璐,穆迎春,韩刚,张洪玉,房洪博,阮志勇4,宋金龙. 磺胺甲恶唑高效降解菌群的多样性分析和降解微生物的分离表征[J]. 生物技术进展, 2021, 11(2): 196-203.
[6]	玄琦月,韩雪,付英梅,. 肺外结核病微生物学诊断方法的研究和应用进展[J]. 生物技术进展, 2021, 11(1): 47-53.
[7]	张兆昆,,周文学,李永丽,,胡建华,,刘占英,. 核黄素发酵菌种改造研究进展[J]. 生物技术进展, 2021, 11(1): 54-60.
[8]	樊英,于晓清,李乐,王晓璐,叶海斌,胡发文,刁菁,刘洪军. 基于16S rRNA高通量测序分析大泷六线鱼表皮粘液及肠道内容物微生物多样性[J]. 生物技术进展, 2021, 11(1): 79-90.
[9]	陈硕,高佳奇,王迪,龙艳,李亮,张晓. DNA四面体纳米结构及其在生物技术领域的应用进展[J]. 生物技术进展, 2020, 10(6): 661-667.
[10]	徐欢欢,张红兵,李会宣,李磊. 常压室温等离子体技术在微生物诱变中的应用进展[J]. 生物技术进展, 2020, 10(4): 358-362.
[11]	李宇邦,吴军林,李曼莎. 微生物发酵处理药食同源植物研究进展[J]. 生物技术进展, 2019, 9(5): 461-466.
[12]	马永凯,陶宏兵,李文茹,谢小保,施庆珊,周少璐. 水性涂料中微生物群落结构及其多样性分析[J]. 生物技术进展, 2019, 9(4): 396-403.
[13]	刘丽辉,蒋慧敏,王佩旋,唐小钰,彭桂香,谭志远. 野生稻内生固氮菌多样性研究进展[J]. 生物技术进展, 2017, 7(6): 567-579.
[14]	袁林喜,张影. 硒超积累植物壶瓶碎米荠的根际微生物特征研究[J]. 生物技术进展, 2017, 7(5): 395-401.
[15]	付丽娜,魏兰芳,王震铄,刘峰,李淼,姬广海. 三七根际微生物群落组成及多样性研究[J]. 生物技术进展, 2017, 7(3): 211-216.

甘露糖赤藓糖醇脂生产及应用研究进展

Research Progress on Production and Application of Mannosylerythritol Lipids

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 4

参考文献 40

相关文章 15

编辑推荐

Metrics

本文评价