关键词: 核黄素, 微生物发酵, 菌种选育, 改造

Abstract: Riboflavin is a kind of water-soluble vitamin, which is closely related to the growth of animals and plants. The human body cannot synthesize riboflavin and needs to absorb it from outside. Therefore, the production of riboflavin has great significant value. This paper introduced the process of riboflavin production, and the microorganisms of riboflavin production. The riboflavin metabolic pathway and mutation breeding of Bacillus subtilis were summarized. The paper mainly introduced the gene recombination methods of Bacillus subtilis, which was to improve the expression of riboflavin operon and to increase the synthetic pathway flux of two precursors (Ru5p and GTP). We also summerized the other new transformation methods in recent years, and looked forward to the future development direction.

Key words: riboflavin, fermentation, strain breeding, transformation