关键词: CHO细胞培养, 细胞改造, 重组抗原表达

Abstract: CHO cell expression system is the preferred system for recombinant glycoprotein production. With the evolving development and applications of serum-free suspension culture technology, genetic engineering and the large- scale culture technologies, CHO cell expression system has become the most important expression or production system of biotechnology products. This system is widely used in the research and production of antibodies, recombinant proteins and vaccines. In recent years, researchers have made great efforts to improve the expression and large-scale production of recombinant proteins by using latest bioengineering technology and the development of the recombinant protein expression mechanism. This article briefly reviewed the recent development of the CHO cell expression system in three aspects: the optimization of the culture medium, construction of engineered CHO strains for high-level production and large-scale culture research, which was expected to provide reference for research and application of CHO cell expression system.

Key words: CHO cell culture, cell engineering, recombinant antigen expression