关键词: 壶瓶碎米荠, 根际微生物, 硒超积累, α-变形菌纲, 硝化螺旋菌纲

Abstract: High-performance 16S rRNA analysis was performed on rhizosphere bacteria from Cardamine hupingshanesis to demonstrate their roles on selenium(Se)-hyperaccumulation by C. hupingshanesis. Compared with the normal soils in study site, there have much higher contents of microorganisms but less microbial diversity in the rhizosphere of C. hupingshanesis, and it was predominant by α-proteobacteria class (15%~22%), β-proteobacteria class (10%~16%), Actinobacteria class (10%~18%), Acidobacteria class (8%~15%), γ-proteobacteria class (5%~16%). Moreover, some special microorganism were characterized with Nitrospira class (2%~5%), Gemmatimonadetes class (2%~5%), Verrucomicrobiae class (2%~4%), Planctomycetacia class (1%~2%) and others (Opitutae class, Sphingobacteria class, Bacilli class, Clostridia class) (3%~4%). Especially, the typical bacteria, such as α-proteobacteria class and Nitrospira class could play important roles during Se-hyperaccumulation for C. hupingshanesis.

Key words: Cardamine hupingshanesis, rhyzosphere bacteria, selenium hyperaccumulation, α-proteobacteria class, Nitrospira class