关键词: 蜂蛹, 多肽, RAW264.7细胞, 免疫活性, 氧化应激

Abstract: Bee pupa polypeptide has attracted wide attention because of its rich nutritional value and biological activities such as immune enhancement, anti-tumor and anti-oxidation. However, there is no report on the immunomodulatory activity of purified components of bee pupa polypeptide in vitro. In order to explore the effect of bee pupa polypeptide on the immune activity of macrophage RAW264.7, BPP-21, a purified component of bee pupa polypeptide, was taken as the research object, and the effects of BPP-21 at different concentrations (12.5, 25, 50, 100 and 200 μg·mL-1) on cell viability, phagocytosis, cytokine secretion, NO secretion and oxidative stress indexes of RAW264.7 were studied. The results showed that BPP-21 had no significant inhibitory activity on RAW264.7 cells within the concentrations ranged from 12.5 to 200 μg·mL-1, and significantly increased nitric oxide (NO) and interferon-γ (IFN-γ) secretion (P<0.05). In the range of 25~200 μg·mL-1, BPP-21 could significantly increase phagocytic activity, as well as interleukin-2 (IL-2) and tumor necrosis factor-α (TNF-α) secretion (P<005). In the range of 50~200 μg·mL-1, the activity of superoxide dismutase (SOD) in cells was significantly increased (P<005). The results showed that BPP-21, a purified component of bee pupa polypeptide, could enhance the immune activity of RAW264.7 macrophages, which provided a theoretical basis for the research and development of bee pupa polypeptide immunomodulator.

Key words: bee pupa, polypeptide, RAW264.7 cells, immune activity, oxidative stress