关键词: 荧光适配体, 孔雀石绿适配体, 生物传感, 荧光成像

Abstract: As a fluorescent probe without labeling, fluorescent aptamer has many potential advantages and has been applied to detect a variety of target substances (such as ATP, RNA), which is currently a hot spot in the field of aptamer research. Malachite green aptamer (MGA) belongs to fluorescent aptamer, which can form binding pockets through ligand-induced folding, thus promoting the luminescence of malachite green (MG). At present, there are few kinds of MGA that have been screened out. Therefore, the characteristics of known MG RNA aptamers and their allosteric bodies, MG DNA aptamers were mainly introduced, as well as the factors affecting the fluorescence intensity of MG-MGA complexes. At the same time, the main MG derivatives and copolymers were also summarized. Finally, the applications of MGA in biosensing and fluorescence imaging were reviewed, and the development direction of MGA was prospected, so as to provide guidance for the application of MGA in biological detection and bioimaging, etc.

Key words: fluorescent aptamer, malachite green aptamer, biosensing, fluorescence imaging