生物技术进展 ›› 2019, Vol. 9 ›› Issue (2): 114-121.DOI: 10.19586/j.2095-2341.2019.0004

• 植物-微生物互作专栏 • 上一篇    下一篇

小麦NTL转录因子的全基因组鉴定及其在小麦与条锈菌互作过程中的表达变化分析

王培1,2,王凤涛2,常洪雷3,冯晶2,郭青云1*,蔺瑞明2*   

  1. 1.青海大学农林科学院, 青海省农林科学院, 农业农村部西宁作物有害生物科学观测实验站; 青海省农业有害生物综合治理重点实验室; 省部共建三江源生态与高原农牧业国家重点实验室, 西宁 810016;
    2.中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室; 农业农村部作物有害生物综合治理重点实验室, 北京 100193;
    3.北京迪福科技有限公司, 北京 101116
  • 收稿日期:2019-01-08 出版日期:2019-03-25 发布日期:2019-02-09
  • 通讯作者: 郭青云，研究员，研究方向为农田杂草综合治理。E-mail：guoqingyunqh@163.com；蔺瑞明，副研究员，研究方向为麦类作物抗病遗传。E-mail：linruiming@caas.cn
  • 作者简介:王培，硕士研究生，研究方向为植物抗病遗传。E-mail：2639911407@qq.com。
  • 基金资助:
    国家重点研发计划项目（2018YFD0200408；2018YFD0200500）资助。

Genome-wide Identification of Wheat NTL Transcription Factors and Their Expression Patterns Analysis in the Wheat-stripe Rust Interaction

WANG Pei, WANG Fengtao, CHANG Honglei, FENG Jing, GUO Qingyun, LIN Ruiming   

  1. 1.Scientific Observation and Experimental Station of Crop Pests in Xining, Ministry of Agriculture and Rural Affairs; Key Laboratory of Agricultural Integrated Pest Management of Qinghai Province; State Key Laboratory of Plateau Ecology and Agriculture of Qinghai University, Qinghai Academy of Agriculture and Forestry Sciences, Academy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, China;
    2.State Key Laboratory for Biology of Plant Diseases and Insect Pests; Key Laboratory of Integrate Pest Management on Crops, Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    3.Beijing Defoe Technology Co. Ltd., Beijing 101116, China
  • Received:2019-01-08 Online:2019-03-25 Published:2019-02-09

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摘要： NTL（NAC with transmembrane motif 1-like，NTM1-LIKE）类转录因子是NAC转录因子家族的一员，其特征是除NAC结构域外，C末端具有跨膜结构域。NTL转录因子已被证实在植物发育以及响应生物和非生物胁迫中发挥着重要作用，但小麦中NTLs的研究相对较少。基于此，对小麦中NTL类转录因子进行全基因组鉴定，并分析NTLs的系统发育、基因结构、保守基序及其在小麦与亲和条锈菌互作过程的表达变化。结果表明，在小麦中有17个NTLs，属于7个同源群，分布在12条染色体上，亚细胞定位的预测分析结果显示，NTL转录因子在细胞核、质膜、叶绿体类囊体及内质网膜都有定位信号的出现。此外，部分NTL转录因子的表达受小麦与条锈菌亲和互作的诱导。研究结果为小麦NTL转录因子家族的功能分析提供了一定的参考。

关键词: 小麦, 条锈病, NAC转录因子, 跨膜结构域

Abstract: NTL (NAC with transmembrane motif 1-like, NTM1-LIKE) transcription factors belong to the NAC transcription factor family, characterized by a transmembrane domain at the C terminus besides the NAC domain. NTLs have been proved to play important roles in plant development and responding to biotic and abiotic stresses, while the studies of NTLs in wheat are relatively less. Based on this situation, genome-wide identification of NTLs in wheat was carried out, and the phylogeny, gene structure, conserved motifs of NTLs and their expression patterns in the wheat-stripe rust interaction were analyzed. The results showed that there were 17 NTLs in wheat, which belonged to 7 homologous groups, and located on 12 chromosomes. Predictive analysis of subcellular localization indicated that NTLs with localization signals generated in nucleus, plasma membrane, chloroplast thylakoid and endoplasmic reticulum. Besides, some NTLs were induced by the infection of stripe rust in compatible interaction. The results of this study provided a reference for the functional analysis of NTL transcription factor family in wheat.

Key words: wheat, stripe rust, NAC transcription factor, transmembrane domain

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