关键词: 硒生物营养强化, 小麦, 发芽, 谷胱甘肽过氧化物酶, 总抗氧化能力

Abstract: The selenium (Se)-biofortified wheat cultivar, Yangmai 16, with low-Se level A (5.245 μg Se/g), high-Se level B (47.850 μg Se/g) and control group CK (0.039 μg Se/g) were selected to germinate, and the germination parameters (root length, sprout length and germination rate), the total Se contents and Se species, and the physiological metabolism activities (AMS, GSH-Px, SOD, T-AOC, H2O2, MDA) were monitored during the different germination phases. The results revealed that there were no differences on root length and sprout length among the three groups, but increased germination rates could be observed with moderate Se contents in wheats. SeMet could be transformed into SeCys2 in high-Se level B during germination. Moreover, the biofortified Se in group A and B could obviously improve the activities of AMS, GSH-Px and SOD, increase the capacity of T-AOC, and reduce the accumulation of H2O2 and MDA.

Key words: selenium biofortification, wheat, germination, GSH-Px, T-AOC