关键词: 酵母双杂交, 技术原理, 应用

Abstract: Yeast two-hybrid system is one of the most powerful and widely used method to detect protein-protein interaction in living cells. Yeast two-hybrid technique is cost-effective, easy to operate, can reach the whole-genome level, and identify the interaction between different varieties. Compared with the traditional detected method, it has obvious advantages and has been applied in more and more fields. In this paper, the yeast two-hybrid technology principle and application were reviewed, including the applications on discovering new protein and proteins new function, setting up the protein linkage map, studing of human genome DNA library, screening target for drug, and so on. The paper was expected to provide reference for the application of yeast two-hybrid system.

Key words: yeast two-hybrid, technical principle, application