高效液相色谱法测定大麦虫磷脂酰胆碱含量

doi:10.3969/j.issn.2095-2341.2014.01.12

生物技术进展 ›› 2014, Vol. 4 ›› Issue (1): 59-62.DOI: 10.3969/j.issn.2095-2341.2014.01.12

高效液相色谱法测定大麦虫磷脂酰胆碱含量

范志影,石冬冬*,常碧影,张萍

中国农业科学院饲料研究所, 北京 100081

收稿日期:2013-10-18 出版日期:2014-01-25 发布日期:2013-12-19
通讯作者: 石冬冬，副研究员，博士，主要从事饲料检测技术研究。Tel:010-82106067;E-mail: shidongdong@caas.cn
作者简介:范志影，副研究员，主要从事饲料检测技术研究。E-mail：fanzhiying@caas. cn
基金资助:
国家现代农业科技城成果惠民科技示范工程（z111100074311016）资助。

Determination of Phosphatidylcholine in Zophobsmorio L. by Liquid Chromatography

FAN Zhi-ying, SHI Dong-dong, CHANG Bi-ying, ZHANG Ping

Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received:2013-10-18 Online:2014-01-25 Published:2013-12-19

摘要/Abstract

摘要： 建立高效液相色谱法测定大麦虫体内磷脂酰胆碱含量的方法。样品经甲醇提取、净化后，经高效液相色谱分析。采用外标法定量，磷脂酸胆碱标准溶液质量浓度在250～2 000 μg/mL范围内呈良好的线性相关，相关系数为0.999 6，样品加标回收率在97%～109%之间，相对标准偏差小于2.4%。对不同生长阶段大麦虫体内磷脂酰胆碱含量测定结果表明，大麦虫体内卵磷脂含量随个体重量的变化符合二项式曲线的变化规律，相关系数R2=0.865(P＜0.01)。

关键词: 大麦虫, 磷脂酰胆碱, 液相色谱

Abstract: A liquid chromatographic method for determination of phosphatidylcholine in Zophobsmorio L. has been developed. The samples were analyzed by liquid chromatography. Quantitative method was external standard method. The linearity was checked by standard solutions at concentration range from 250～2 000 μg/mL, the correlation coefficient was 0.999 6. The recovery values ranged from 97% to 109% with relative standard deviations(RSDs) no larger than 2.4%. For different growth stage of Zophobsmorio L., the results of the content of phosphatidylcholine in vivo showed that the content of phosphatidylcholine was in line with the binomial curve along with the change of the weight, the correlation coefficient R2 was 0.865 (P<0.01).

Key words: liquid chromatography, phosphatidylcholine, Zophobsmorio L.

范志影,石冬冬,常碧影,张萍. 高效液相色谱法测定大麦虫磷脂酰胆碱含量[J]. 生物技术进展, 2014, 4(1): 59-62.

FAN Zhi-ying, SHI Dong-dong*, CHANG Bi-ying, ZHANG Ping. Determination of Phosphatidylcholine in Zophobsmorio L. by Liquid Chromatography[J]. Curr. Biotech., 2014, 4(1): 59-62.

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高效液相色谱法测定大麦虫磷脂酰胆碱含量

Determination of Phosphatidylcholine in Zophobsmorio L. by Liquid Chromatography

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