关键词: 芦笋茎秆, 芦丁, 槲皮素, 白藜芦醇, 高效液相色谱

Abstract: In order to establish a HPLC method to determinate its content of rutin, quercetin and resveratrol. The study used different methods to extract rutin, quercetin and resveratrol from asparagus stems, the results showed that methanol, methanol-HCl and ethanol had the best extraction rate for rutin, quercetin and resveratrol extraction. And it chose the optimum chromatographic conditions of HPLC method: The mobile phases of rutin and quercetin were 0.2 mol/L sodium acetate-methanol (Me-OH∶H2O=35∶65) solution (pH 2.80 adjusted with phosphoric acid), anhydrous methanol-0.4% phosphoric acid solution (55∶45, V/V), isocratic elution, and resveratrol mobile phase was water and methanol, binary linear gradient elution, respectively. Their detection wavelength was 254 nm, 360 nm and 306 nm, and velocity of flow was 0.8 mL/min, 1.0 mL/min and 0.8 mL/min. The average recoveries of rutin, quercetin and resveratrol were 98%, 97% and 98%.

Key words: asparagus stems, rutin, quercetin, resveratrol, HPLC