关键词: 硒形态分析, 硒代氨基酸, 液相色谱-原子荧光光谱（LC-AFS）, 高效液相色谱与电感耦合等离子质谱（HPLC-ICP-MS）, 同步辐射X射线微区分析（STXM）

Abstract: Based on recent researches on selenium (Se) speciation analysis on biological samples, the modified extraction methods on Se speciation and composition from high Se contents samples (>10 mg Se/kg) and low Se contents samples (<1 mg Se/kg) were summarized, respectively. The liquid chromatography-atomic fluorescence spectrometry (LC-AFS) and high performance liquid chromatography-inductively coupled plasma-mass spectrometry (HPLC-ICP-MS) employed to separate and determine the Se speciation and composition were discussed. Furthermore, the synchrotron radiation scanning transmission X-ray microscopy (STXM) method was reviewed and prospected on total Se and Se speciation distributions in situ on bacteria samples.

Key words: selenium speciation analysis, selenoaminoacid, liquid chromatography-atomic fluorescence spectrometry (LC-AFS), high performance liquid chromatography-inductively coupled plasma-mass spectrometry (HPLC-ICP-MS), synchrotron radiation scanning transmission X-ray microscopy (STXM)