生物技术进展 ›› 2017, Vol. 7 ›› Issue (1): 85-89.DOI: 10.3969/j.issn.2095-2341.2017.01.14

• 技术与方法 • 上一篇    

高效液相色谱法测定转化液中海藻糖

宋金松,马春燕,赵伟*,段莹莹,方建,曹大鹏   

  1. 山东福洋生物科技有限公司, 山东 德州 253100
  • 收稿日期:2016-03-23 出版日期:2017-01-25 发布日期:2016-07-21
  • 通讯作者: 赵伟，工程师，研究方向为生物化学。E-mail：xiaoyanzizhw@aliyun.com
  • 作者简介:宋金松，中级工程师，研究方向为微生物发酵。E-mail:pysongzhen@163.com。

Determination of Trehalose in Transformation Liquid by High Performance Liquid Chromatography

SONG Jinsong, MA Chunyan, ZHAO Wei, DUAN Yingying, FANG Jian, CAO Dapeng   

  1. Shandong Fuyang Biotechnology Co., Ltd., Shandong Dezhou 253100, China
  • Received:2016-03-23 Online:2017-01-25 Published:2016-07-21

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摘要： 以麦芽糖为反应底物，与从土壤中筛选的产海藻糖合酶菌株发酵制得的粗酶液反应制取转化液。采用高效液相色谱法以Sugar-PakTM Ⅰ为色谱柱测定转化液中海藻糖的含量。研究结果显示，该方法能够将海藻糖和葡萄糖完全分开，与DNS比色法所得结果一致，为1.5 g/100mL，但HPLC法在测定海藻糖含量时，操作过程及数据处理更为简单，易于掌握。

关键词: 海藻糖, 高效液相色谱法, 葡萄糖

Abstract:

Transformation liquid was obtained from the reaction of crude enyme with maltose as oligomer. The crude enyme was fermented by trehalose synthase producing strain which was selected from soil. The concentration of trehalose in the transformation was determined by HPLC with Sugar-PakTM Ⅰ. Results showed that the method could separate the trehalose and glucose completely, accordance with the result of DNS colorimetric method of 1.5g/100mL, but the HPLC operation process and data processing were simpler and easier to control in trehalose content determination.

Key words: trehalose, high performance liquid chromatography, glucose

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