生物技术进展 ›› 2013, Vol. 3 ›› Issue (6): 433-438.DOI: 10.3969/j.issn.2095-2341.2013.06.11

• 分离与检测 • 上一篇    下一篇

大孔树脂对红薯叶总黄酮的吸附及解吸特性研究

赵玉芬1,李佩艳2,3*,郑小林3,尹飞4   

  1. 1.山东省东营市河口区实验中学, 山东 东营 257200； 2.河南科技大学食品与生物工程学院， 河南 洛阳471023； 3.浙江工商大学食品与生物工程学院， 浙江省果蔬保鲜与加工技术研究重点实验室 ， 杭州 310035; 4.河南科技大学农学院， 河南 洛阳 471023
  • 收稿日期:2013-10-08 出版日期:2013-11-25 发布日期:2013-11-04
  • 通讯作者: 李佩艳，讲师，博士，主要从事食品质量与安全控制、果蔬贮藏与保鲜等方面研究。E-mail: lipeiyan77@163.com
  • 作者简介:赵玉芬，本科，生物学专业。
  • 基金资助:
    河南科技大学科学研究基金项目（2008QN028）；河南科技大学大学生 研究训练计划（SRTP）项目（2011118）资助。

Absorption and Desorption of Total Flavonoids from Sweet Potato Leaves with Macroporous Resins

ZHAO Yu-fen, LI Pei-yan, ZHENG Xiao-lin, YIN Fei4   

  1. 1.Shandong Dongying Hekou District Experimental School， Shandong Dongying 257200, China； 2.College of Food and Bioengineering, Henan University of Science and Technology, Henan Luoyang 471023, China； 3.College of Food and Bioengineering, Zhejiang Gongshang University, Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, Hangzhou 310035, China; 4.College of Agronomy, Henan University of Science and Technology, He nan Luoyang 471023, China
  • Received:2013-10-08 Online:2013-11-25 Published:2013-11-04

PDF

摘要： 以红薯叶为原料，选用D101、AB-8、DM130大孔吸附树脂进行静态吸附及解 吸试验，筛选出适合吸附分离红薯叶总黄酮的树脂并确立其最佳吸附及解吸工艺参 数。试验结果表明，AB\|8大孔吸附树脂对红薯叶总黄酮吸附、解吸效果较好，最佳 吸附条件为上样液浓度0.3 mg/mL，上样液流速2 mL/min，上样液pH 5.0，吸附率为 66.8%；最佳解吸条件为洗脱液浓度90%，洗脱液流速4 mL/min，洗脱液用量5 BV， 解吸率为70.3%。

关键词: 红薯叶, 总黄酮, 大孔吸附树脂, 吸附, 解吸

Abstract: Static adsorption and desorption capacity were assayed to screen the proper macroporous resin for total flavonoids from sweet potato leaves, and most suitable manipulation parameters of macroporous resin were confirmed. The results showed that AB-8 macroporous resin had strong adsorption and desorption capacity among the three resins used in this experiment. The most appropriate adsorption conditions were: sample concentration of 0.3 mg/mL, sample flow velocity of 2 mL/min, pH 5.0, and the adsorption rate reached 66.8%. The most fitting elution conditions were: ethanol concentration of 90%, elution rate of 4 mL/min, elution volume 5 BV, and the desorption rate was 70.3%.

Key words: sweet potato leaves, total flavanoids, macroporous resin, adsorption, desorption

版权所有 © 2021《生物技术进展》编辑部

京ICP备07026971-2 　　 京公网安备11010802021232号

本系统由北京玛格泰克科技发展有限公司设计开发 技术支持：support@magtech.com.cn