关键词: 白背三七, 组织培养, 总黄酮含量提高, 试管苗

Abstract: The test mainly aimed at the establishment of a tissue culture technology to improve the total flavonoids of Gynura divaricate callus. The tissue culture technology was used to change the MS medium with different hormone combination, carbon source, nitrogen source and trace element ratio. The orthogonal experiment results showed that the maximum total flavones content was increased by 15% when the NAA∶6-BA was 0.1 mg/L∶1.5 mg/L, carbon source was 30 g/L, Mn2+∶Zn2+ was 44.6 mg/L∶13.2 mg/L and nitrogen source KNO3∶NH4NO3 was 2 020 mg/L∶1 600 mg/L. A system of tissue culture was developed for the enhancing of the total flavonoid of the G. divaricata DC, which was helpful for the seeding cultivation technology of G. divaricata DC and provided essential principles and techniques of improving the secondary metabolism for further research.

Key words: Gynura divaricata（L.）DC, tissue culture technology, total flavonoids content, callus