关键词: 桑叶, 超临界萃取技术, 总黄酮得率, 正交实验法

Abstract: The intent of this study is to explore the best solutions for the supercritical CO2 extraction of flavonoids from mulberry leaves. This study used supercritical carbon dioxide extraction technology, detected the effects of extraction pressure, extraction temperature, extraction time, entrainer flow on the yield of total flavonoids in mulberry leaves through orthogonal experiment method, to optimize the best process conditions. Results showed that: orthogonal experiment for the optimum extraction conditions were as follows: extraction pressure was 25 MPa, extraction temperature was 40 ℃, extraction time was 3 h, entrainer anhydrous ethanol velocity was 2.5 mL/min. The expected flavonoids yield rate was 6.19%±0.26%, the repeatability was good. Flavonoids extraction rate by the supercritical carbon dioxide extraction from mulberry leaves was high. This study provided theoretical and experimental basis for further development of mulberry leaf.

Key words: mulberry, supercritical carbon dioxide extraction, flavonoid yield, thogonal experiment method