生物酶活性测试技术研究进展

doi:10.19586/j.2095-2341.2024.0158

生物技术进展 ›› 2025, Vol. 15 ›› Issue (1): 58-66.DOI: 10.19586/j.2095-2341.2024.0158

生物酶活性测试技术研究进展

侯晓妮(), 刘明东, 吕昊, 叶德萍, 马丽侠, 周李华()

中国测试技术研究院生物研究所，成都 610023

收稿日期:2024-09-29 接受日期:2024-11-13 出版日期:2025-01-25 发布日期:2025-03-07
通讯作者: 周李华
作者简介:侯晓妮 E-mail：houxn1998@126.com；
基金资助:
国家重点研发计划项目(2021YFF0600802)

Research Progress on Measuring Technology of Bio-enzyme Activity

Xiaoni HOU(), Mingdong LIU, Hao LYU, Deping YE, Lixia MA, Lihua ZHOU()

Institute of Biology，National Institute of Measurement and Test Technology，Chengdu 610023，China

Received:2024-09-29 Accepted:2024-11-13 Online:2025-01-25 Published:2025-03-07
Contact: Lihua ZHOU

摘要/Abstract

摘要：

生物酶在工业、农业、医药、环境保护等领域生产中发挥着巨大的作用。活性作为生物酶的主要性能参数，直接影响着生物酶的质量和应用。随着科学技术的发展，生物酶活性检测也产生了许多新技术新方法。回顾了国内外生物酶活性主流的测试技术和方法，包括光谱法、电化学法、色谱法、放射性标记法、质谱法、表面等离子体共振法、表面增强拉曼光谱法，综述了不同方法的基本原理、适用条件及优缺点,并提出了生物酶活性测试结果的准确性和可靠性的建议，以期对生物酶活性检测、方法选取、质量控制和产品应用提供指导。

关键词: 生物酶, 活性, 光谱法, 电化学法, 色谱法, 质谱法, 表面等离子体共振法, 表面增强拉曼光谱法

Abstract:

Biological enzymes play significant roles in the agriculture， industrial production， medicine and environment protection. Activity is the main performance parameter of bio-enzymes， and directly affects the quality and application of biological enzymes. With the development of science and technology， the detection methods of biological enzyme activity have also produced many new technologies and new methods. This paper reviewed the mainstream test techniques and methods of biological enzyme activity at home and abroad， including spectroscopy， electrochemical， chromatography， mass spectrometry， surface plasmon resonance， surface enhanced Raman spectroscopy. We also introduced the basic principles， applicable conditions and advantages and disadvantages of different methods. Finally， some suggestions were put forward for the accuracy and reliability of the test results of biological enzyme activity. The paper was expected to provide guidance on biological enzyme activity detection， method selection， quality control and product application.

Key words: bio-enzyme, activity, photometry, electrochemical method, chromatography, mass spectrometry, surface plasmon resonance, surface enhanced Raman spectroscopy

中图分类号:

侯晓妮, 刘明东, 吕昊, 叶德萍, 马丽侠, 周李华. 生物酶活性测试技术研究进展[J]. 生物技术进展, 2025, 15(1): 58-66.

Xiaoni HOU, Mingdong LIU, Hao LYU, Deping YE, Lixia MA, Lihua ZHOU. Research Progress on Measuring Technology of Bio-enzyme Activity[J]. Current Biotechnology, 2025, 15(1): 58-66.

图/表 2

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生物酶活性测试技术研究进展

Research Progress on Measuring Technology of Bio-enzyme Activity

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