贝莱斯芽孢杆菌联合含钙土壤调理剂对烟草青枯病的防治

doi:10.19586/j.2095-2341.2024.0064

生物技术进展 ›› 2024, Vol. 14 ›› Issue (5): 839-847.DOI: 10.19586/j.2095-2341.2024.0064

• 研究论文 • 上一篇

贝莱斯芽孢杆菌联合含钙土壤调理剂对烟草青枯病的防治

王博文¹(), 王金星², 邱成尧², 秦庆宪², 王菊香¹, 姚博², 李红艳², 谭官鑫², 杨应伟²(), 姬广海¹()

^1.云南农业大学植物保护学院，昆明 650201
^2.云南省烟草公司普洱市公司，云南普洱 665000

收稿日期:2024-03-28 接受日期:2024-05-22 出版日期:2024-09-25 发布日期:2024-10-22
通讯作者: 杨应伟,姬广海
作者简介:王博文 E-mail: l481623593@qq.com；
基金资助:
景谷烟区烟草青枯病综合防控技术研究与应用(PEYCKY202201)

Control of Tobacco Bacterial Wilt by Bacillus velezensis Combined with Calcium-containing Soil Conditioner

Bowen WANG¹(), Jinxing WANG², Chengyao QIU², Qingxian QIN², Juxiang WANG¹, Bo YAO², Hongyan LI², Guanxin TAN², Yingwei YANG²(), Guanghai JI¹()

^1.College of Plant Protection，Yunnan Agricultural University，Kunming 650201，China
^2.Yunnan Province Tobacco Company Puer Company，Yunnan Tobacco Company，Yunnan Puer 665000，China

Received:2024-03-28 Accepted:2024-05-22 Online:2024-09-25 Published:2024-10-22
Contact: Yingwei YANG,Guanghai JI

摘要/Abstract

摘要：

烟草是重要的经济作物，烟草青枯病对烟草产业造成了极大的经济损失。因此需要一种安全有效的方法防止烟草青枯病的发生。前期研究中发现可溶性钙肥和生防菌均能有效抑制青枯病菌的生长。在田间种植烟草时，通过单施或组合施用不同的含钙土壤调理剂和生防菌，筛选防治烟草青枯病的最佳组合；采集不同处理的烟草根际土，运用荧光定量PCR和高通量测序技术，探究不同处理下烟草根际土壤中青枯菌数量的变化以及微生物群落组成和结构的变化。田间试验表明，土壤调理剂和生防菌的施用均能有效降低烟草青枯病的发生，但3种含钙调理剂中只有硝酸铵钙能更好地与生防菌GT11复合。该组合处理能降低土壤中青枯病菌的数量，改善烟草根际土壤微生物的群落组成与结构，显著降低烟草青枯病的发病率和病情指数，田间防效达60%。研究证实硝酸铵钙与贝莱斯芽孢杆菌GT11的联合施用能够有效防治烟草青枯病，为烟草生产中青枯病的防治提供了新的思路和理论依据。

关键词: 硝酸铵钙, 贝莱斯芽孢杆菌, 烟草青枯病, 生物防治

Abstract:

Tobacco is one of the most important cash crops， and bacterial wilt of tobacco causes a great loss to tobacco production. Therefore， a safe and effective method is needed to prevent the occurrence of tobacco bacterial wilt. In the previous study， it found that the soluble calcium fertilizer could effectively control tobacco bacterial wilt. When tobacco was planted in the field， the best combination was selected by applying different soil conditioner containing calcium and biocontrol bacteria. The quantity of Ralstonia solanacearum was quantified throughfluorescence quantitative PCR. The composition and structure of microbial community in tobacco rhizosphere soil under different treatments were studied by high-throughput sequencing. Field experiments showed that the application of soil conditioner and biocontrol agent could effectively reduce the occurrence of tobacco bacterial wilt， but only ammonium calcium nitrate could compound with biocontrol bacteria GT11 better. The combination treatment decreased the quantity of bacterial wilt in soil， improved the composition and structure of microbial community in tobacco rhizosphere soil， and significantly reduced the incidence and disease index of tobacco bacterial wilt. The control effect in the field was up to 60%. The results showed that the combination of calcium ammonium nitrate and Bacillus velezensis GT11 could effectively control tobacco bacterial wilt， which provided a new idea and theoretical basis for tobacco production.

Key words: calcium ammonium nitrate, Bacillus velezensis, tobacco bacterial wilt, biological control

中图分类号:

S435.72

王博文, 王金星, 邱成尧, 秦庆宪, 王菊香, 姚博, 李红艳, 谭官鑫, 杨应伟, 姬广海. 贝莱斯芽孢杆菌联合含钙土壤调理剂对烟草青枯病的防治[J]. 生物技术进展, 2024, 14(5): 839-847.

Bowen WANG, Jinxing WANG, Chengyao QIU, Qingxian QIN, Juxiang WANG, Bo YAO, Hongyan LI, Guanxin TAN, Yingwei YANG, Guanghai JI. Control of Tobacco Bacterial Wilt by Bacillus velezensis Combined with Calcium-containing Soil Conditioner[J]. Current Biotechnology, 2024, 14(5): 839-847.

图/表 8

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处理编号	具体处理方式
T	碳酸氢铵+氧化钙
S	硅钙肥
N	硝酸铵钙
B	生物有机肥GT11
BT	生物有机肥GT11+碳酸氢铵+氧化钙
BS	生物有机肥GT11+硅钙肥
BN	生物有机肥GT11+硝酸铵钙
CK	空白对照

处理编号	具体处理方式
T	碳酸氢铵+氧化钙
S	硅钙肥
N	硝酸铵钙
B	生物有机肥GT11
BT	生物有机肥GT11+碳酸氢铵+氧化钙
BS	生物有机肥GT11+硅钙肥
BN	生物有机肥GT11+硝酸铵钙
CK	空白对照

处理	发病率/%	病情指数	相对防效/%
T	70.00±2.88 b	9.63±0.19 bcd	46.70±1.03 abc
S	60.00±0 cd	8.33±0.32 cd	53.86±1.77 ab
N	52.50±4.33 de	8.05±1.12 cd	55.36±6.21 ab
B	65.00±0 bc	8.89±0.96 bcd	50.77±5.34 abc
BT	70.00±2.89 b	10.00±0.32 bc	44.63±1.79 bc
BS	65.00±2.89 bc	11.11±1.28 b	38.48±7.1 c
BN	46.67±4.41 e	7.72±0 d	60.00±0.02 a
CK	87.50±1.44 a	18.06±0.8 a	—

处理	发病率/%	病情指数	相对防效/%
T	70.00±2.88 b	9.63±0.19 bcd	46.70±1.03 abc
S	60.00±0 cd	8.33±0.32 cd	53.86±1.77 ab
N	52.50±4.33 de	8.05±1.12 cd	55.36±6.21 ab
B	65.00±0 bc	8.89±0.96 bcd	50.77±5.34 abc
BT	70.00±2.89 b	10.00±0.32 bc	44.63±1.79 bc
BS	65.00±2.89 bc	11.11±1.28 b	38.48±7.1 c
BN	46.67±4.41 e	7.72±0 d	60.00±0.02 a
CK	87.50±1.44 a	18.06±0.8 a	—

处理	pH	有机质/(g·kg^-1)	水解性氮/(mg·kg^-1)	有效磷/(mg·kg^-1)	速效钾/(mg·kg^-1)	交换性钙/(mg·kg^-1)
NH	5.49±0.03 a	17.67±0.47 d	95.33±3.05 d	75.97±1.16 b	413.33±6.81 d	832.33±12.67 c
ND	5.33±0.07 b	23.07±0.55 c	113.33±4.16 b	62.93±1.46 d	333.33±12.86 e	493.67±8.08 e
BH	5.50±0.03 a	22.50±0.61 c	103.00±2.00 c	82.37±0.99 a	689.00±8.19 a	905.67±7.02 b
BD	5.29±0.04 b	22.80±0.30 c	107.00±2.00 bc	71.67±0.72 c	455.00±7.00 c	733.00±12.00 d
CKH	5.30±0.04 b	24.80±0.35 b	96.33±1.52 d	46.63±1.18 f	631.67±10.69 b	935.33±7.51 ab
CKD	5.14±0.06 c	30.67±0.40 a	136.33±5.51 a	60.13±0.94 e	444.00±34.66 cd	722.00±19.97 d
YS	5.04±0.03 d	25.30±0.10 b	141.00±2.00 a	38.70±0.75 g	122.00±11.52 f	958.57±31.34 a

贝莱斯芽孢杆菌联合含钙土壤调理剂对烟草青枯病的防治

Control of Tobacco Bacterial Wilt by Bacillus velezensis Combined with Calcium-containing Soil Conditioner

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处理	Chao1指数	Ace指数	Shannon指数	Simpson指数	覆盖度
S	205.00±4.16a	205.00±4.16a	4.34±0.67a	0.18±0.01c	1
N	179.30±7.13b	179.33±7.13b	3.68±0.16b	0.25±0.03abc	1
B	124.30±5.24c	124.33±5.24c	2.77±0.81c	0.28±0.01ab	1
BN	203.30±5.36a	203.33±5.36a	3.73±0.24b	0.27±0.05ab	1
CKH	179.00±7.00b	179.00±7.00b	3.82±0.57b	0.22±0.02bc	1
CKD	163.00±6.66b	163.00±6.66b	3.17±0.65c	0.33±0.02a	1

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