关键词: 酵母, GS115, 基因组提取, 溶壁酶

Abstract: Extraction genomic DNA from yeasts for some assays is very important. Yeast-lysis solution contained 0.2 mol/L lithium acetate, 1% SDS and other components can efficiently lysis Pichia pastoris GS115 cells. In comparison with two kinds of yeast genomic DNA extraction kits, the amount of genomic DNA extracted by this way was more than five times as much as those by the kits from same volume liquid culture. Moreover, the genomic DNA extraction protocol set up in the paper was simple and easy to perform. It took 2 hours to finish a whole protocol. To further test the quality of genomic DNA, PCR amplification and Southern blot analysis of AOX gene in yeast genomic DNA were done. Therefore, we have developed a quick, lowcost and effective genomic DNA extraction protocol from yeast cells in this paper.