新型冠状病毒奥密克戎感染下血小板转录组与蛋白质组的对比分析

doi:10.19586/j.2095-2341.2024.0032

生物技术进展 ›› 2024, Vol. 14 ›› Issue (4): 649-656.DOI: 10.19586/j.2095-2341.2024.0032

• 研究论文 • 上一篇

新型冠状病毒奥密克戎感染下血小板转录组与蛋白质组的对比分析

马叶子¹^,²(), 夏美娟¹^,², 刘翠翠¹^,², 王洪涛¹^,², 周家喜¹^,²()

^1.中国医学科学院血液病医院（中国医学科学院血液学研究所）北京协和医学院，血液与健康全国重点实验室，国家血液系统疾病临床医学研究中心，细胞生态海河实验室，天津 300020
^2.天津医学健康研究院，天津 301600

收稿日期:2024-02-27 接受日期:2024-04-22 出版日期:2024-07-25 发布日期:2024-08-07
通讯作者: 周家喜
作者简介:马叶子E-mail: mayezi@ihcams.ac.cn；
基金资助:
中国医学科学院医学与健康科技创新工程项目(2021-I2M-1-073);天津市科技计划青年项目(22JCQNJC01270)

Comparative Analysis of Platelet Transcriptome and Proteome Changes in SARS-CoV2 Omicron Infection

Yezi MA¹^,²(), Meijuan XIA¹^,², Cuicui LIU¹^,², Hongtao WANG¹^,², Jiaxi ZHOU¹^,²()

^1.State Key Laboratory of Experimental Hematology，National Clinical Research Center for Blood Diseases，Haihe Laboratory of Cell Ecosystem，Institute of Hematology & Blood Diseases Hospital，Chinese Academy of Medical Sciences & Peking Union Medical College，Tianjin 300020，China
^2.Tianjin Institutes of Health Science，Tianjin 301600，China

Received:2024-02-27 Accepted:2024-04-22 Online:2024-07-25 Published:2024-08-07
Contact: Jiaxi ZHOU

摘要/Abstract

摘要：

血小板是维持机体稳态和应对疾病感染等的关键血液成分，其无核特征以及转录本和蛋白质来源的多样性导致血小板的转录组和蛋白组相关性普遍偏低。前期研究已解析了血小板蛋白质组在新型冠状病毒（severe acute respiratory syndrome coronavirus 2,SARS-CoV2）奥密克戎感染不同阶段的动态表达模式，但对其具体来源未作深入分析。为了解血小板对奥密克戎感染的应答机制，对血小板蛋白组与转录本进行了对比分析，以探究不同蛋白分子的来源。结果发现，血小板转录组变化相较于蛋白组更为剧烈，且二者的变化存在差异。感染后血小板转录组与蛋白组共同上调的基因/蛋白主要富集了固有免疫、抗病毒免疫反应等特征，提示相关蛋白可能来自血小板内RNA翻译。血小板蛋白组单独上调的蛋白特征包括急性炎症反应、吞噬识别作用等，提示该部分蛋白可能由血浆直接摄入或与其他细胞互作所得。此外，血小板转录组也单独上调RNA代谢过程、RNA剪接的调控等相关基因。研究结果为探究奥密克戎感染条件下血小板RNA和蛋白质的来源多样性提供了重要的数据支撑。

关键词: 奥密克戎, 血小板, 转录组, 蛋白组

Abstract:

As an important blood component， platelets play a key role in the maintenance of homeostasis and the stress of diseases infection. The absence of nuclei and the diversity of transcripts and protein sources lead to a generally low transcriptomic and proteomic correlation of platelets. Previous research have analyzed the dynamic expression patterns of platelet proteome at different stages of severe acute respiratory syndrome coronavirus 2 (SARS-CoV2） Omicron infection， but the specific sources have not been thoroughly analyzed. In order to further understand the mechanism of platelet response in Omicron infection， we compared the platelet proteome with the transcript to explore the sources of different protein molecules. The results showed that the changes of platelet transcriptome were more drastic than those of proteome， and the changes of the two were different. The genes/proteins co-upregulated by platelet transcriptome and proteome after infection mainly enriched innate immunity and antiviral immune response， suggesting that related proteins may be derived from RNA translation in platelets. The upregulation features of platelet proteome included acute inflammatory response and phagocytosis recognition， suggesting that this part of the protein may be obtained by direct plasma intake or interaction with other cells. In addition， platelet transcriptome also independently up-regulated RNA metabolism and RNA splicing regulation and other related genes. This study provides important data support for exploring the diversity of platelet RNA and protein sources under Omicron infection.

Key words: Omicron, platelets, transcriptome, proteome

中图分类号:

Q939.47

马叶子, 夏美娟, 刘翠翠, 王洪涛, 周家喜. 新型冠状病毒奥密克戎感染下血小板转录组与蛋白质组的对比分析[J]. 生物技术进展, 2024, 14(4): 649-656.

Yezi MA, Meijuan XIA, Cuicui LIU, Hongtao WANG, Jiaxi ZHOU. Comparative Analysis of Platelet Transcriptome and Proteome Changes in SARS-CoV2 Omicron Infection[J]. Current Biotechnology, 2024, 14(4): 649-656.

图/表 5

表1 数据来源信息

Table 1 Data source information

组别	健康供者		奥密克戎急性期患者
组别	蛋白组(n=35)	转录组(n=26)	蛋白组(n=27)	转录组(n=30)
性别:女/%	15/(42.9)	9/(34.6)	16/(59.2)	16/(53.3)
年龄平均值	41.9±9.3	39.3±9.2	41.3±13.4	40.2±12.8
年龄范围	25.0~64.0	25.0~57.0	16.0~67.0	16.0~67.0

图1 基于蛋白质组分析奥密克戎感染下血小板抗病毒能力的变化A：奥密克戎患者与健康供者血小板蛋白组的PCA降维结果；B：奥密克戎患者与健康供者相比血小板蛋白组的差异蛋白；红色表示表达水平上调的蛋白(P-adj<0.05，log2FC>0.25)；蓝色表示表达水平下调的蛋白(P-adj<0.05，log2FC<-0.25)；灰色表示表达水平无显著差异的蛋白；C：感染后血小板蛋白组上调和下调蛋白的代表性生物学过程；D：箱线图展示奥密克戎感染血小板蛋白组下调的特征蛋白表达水平；E：箱线图展示奥密克戎感染血小板蛋白组上调的特征蛋白的表达水平；**、***分别表示在P<0.01、P<0.001水平上具有统计学意义。

Fig. 1 The change of ability response to virus of platelets after Omicron infection based on proteornics analysis

图2 基于转录组分析奥密克戎感染条件下血小板激活与免疫反应能力的变化A：奥密克戎患者与健康供者血小板转录组的PCA降维结果；B：奥密克戎患者与健康供者相比血小板转录组的差异基因；红色表示表达水平上调的基因(P-adj<0.05，log2FC>0.25)；蓝色表示表达水平下调的基因(P-adj<0.05, log2FC<-0.25)；灰色表示表达水平无显著差异的基因；C：感染后血小板转录组上调和下调基因的代表性生物学过程；D：箱线图展示奥密克戎感染血小板转录组下调的特征基因表达水平；E：箱线图展示奥密克戎感染血小板转录组上调的特征基因表达水平；**、***分别表示在P<0.01、P<0.001水平上具有统计学意义。

Fig. 2 The change of platelet activation and immune response after Omicron infection based on transcriptomic analysis

图3 血小板转录组与蛋白质组的共同上调基因A：韦恩图展示血小板转录组与蛋白组的共同基因交集数量；B：Upset展示血小板转录组与蛋白组共同检测到的基因在转录组与蛋白质组中的变化；C：柱状图展示转录组与蛋白质组共同上调基因的代表性生物学过程；D：柱状图展示转录组与蛋白组均上调的基因或蛋白交集的基因及差异倍数

Fig. 3 The co-upregulated genes of platelet transcriptome and proteome

图4 血小板转录组与蛋白质组存在不一致的变化A：仅在血小板转录组中表达上调、下调和无显著变化基因所占的比例；B：仅在血小板转录组中上调基因的代表性生物学过程；C：仅在血小板蛋白组中表达上调、下调和无显著变化蛋白所占的比例；D：仅在血小板蛋白质组中上调蛋白的代表性生物学过程

Fig. 4 Inconsistent changes in platelet transcriptome and proteome

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新型冠状病毒奥密克戎感染下血小板转录组与蛋白质组的对比分析

Comparative Analysis of Platelet Transcriptome and Proteome Changes in SARS-CoV2 Omicron Infection

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