生长素信号途径参与南瓜白粉菌胁迫的响应研究

doi:10.19586/j.2095-2341.2023.0162

生物技术进展 ›› 2024, Vol. 14 ›› Issue (3): 433-441.DOI: 10.19586/j.2095-2341.2023.0162

• 研究论文 • 上一篇

生长素信号途径参与南瓜白粉菌胁迫的响应研究

张慧¹(), 刘愽愽¹, 李凤梅¹(), 崔健²()

^1.青岛科技大学海洋科学与生物工程学院，山东青岛 266042
^2.青岛市农业科学研究院，山东青岛 266199

收稿日期:2023-12-14 接受日期:2024-01-24 出版日期:2024-05-25 发布日期:2024-06-18
通讯作者: 李凤梅,崔健
作者简介:张慧 E-mail： 824572941@qq.com；
基金资助:
国家自然科学基金项目(32072654)

Study on the Involvement of Auxin Signaling Pathway in Response to Powdery Mildew Stress in Pumpkin

Hui ZHANG¹(), Bobo LIU¹, Fengmei LI¹(), Jian CUI²()

^1.College of Marine Science and Biological Engineering，Qingdao University of Science & Technology，Shandong Qingdao 266042，China
^2.Qingdao Institute of Agricultural Science Research，Shandong Qingdao 266199，China

Received:2023-12-14 Accepted:2024-01-24 Online:2024-05-25 Published:2024-06-18
Contact: Fengmei LI,Jian CUI

摘要/Abstract

摘要：

生长素（auxin, IAA）信号途径在植物生长、生物胁迫和非生物胁迫响应中发挥着重要的作用。白粉病是南瓜普遍发生且较为严重的一种病害，为探究IAA信号途径响应白粉病胁迫的分子机制，对白粉菌处理的南瓜叶片进行了转录组测序和全基因组DNA甲基化测序分析。结果发现，在IAA信号途径中，有25个差异表达基因，53个基因发生了差异甲基化，其中16个生长素上调小RNA (SAUR)基因均发生了不同程度的甲基化，说明这些基因可能参与了白粉病胁迫响应。SAUR50（CmoCh19G007170）基因的甲基化水平降低，且甲基化区域位于基因的启动子区。在白粉病胁迫下SAUR50的表达水平显著上调，在IAA诱导下显著下调，因此该基因可能通过DNA甲基化调节其表达水平，并且通过IAA信号途径参与南瓜白粉病胁迫的调控。研究结果为IAA信号途径响应白粉病胁迫途径与抗白粉病南瓜分子育种提供了理论依据。

关键词: 白粉病, 生长素信号途径, DNA甲基化, 转录组

Abstract:

Auxin （IAA） signaling pathway plays an important role in plant growth and response to biological and abiotic stress. Powdery mildew （PM） is a common and serious disease in pumpkin. In order to explore the molecular mechanism of IAA signaling pathway in response to PM stress， transcriptomic sequencing and whole genome DNA methylation sequencing were performed on pumpkin leaves treated with PM. As a result， we found 25 differentially expressed genes in the IAA signaling pathway. Fifty-three genes were differentially methylated， among which 16 austerin-upregulated small RNA （SAUR） genes were methylated to different degrees， suggesting that these genes may be involved in the response to powdery mildew stress. The methylation level of SAUR50 （CmoCh19G007170） gene was reduced， and the methylation region was located in the promoter region of the gene. The expression level of SAUR50 was significantly up-regulated under PM stress and significantly down-regulated under IAA induction. Therefore， this gene may regulate its expression level through DNA methylation and participate in the regulation of PM stress in pumpkin through IAA signaling pathway. The results provided a theoretical basis for IAA signaling pathway in response to powdery mildew stress molecular breeding of pumpkin resistant to PM.

Key words: powdery mildew, auxin signaling pathway, DNA methylation, transcriptome

中图分类号:

Q946.885+.1

张慧, 刘愽愽, 李凤梅, 崔健. 生长素信号途径参与南瓜白粉菌胁迫的响应研究[J]. 生物技术进展, 2024, 14(3): 433-441.

Hui ZHANG, Bobo LIU, Fengmei LI, Jian CUI. Study on the Involvement of Auxin Signaling Pathway in Response to Powdery Mildew Stress in Pumpkin[J]. Current Biotechnology, 2024, 14(3): 433-441.

图/表 6

参考文献 37

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基因 ID	log₂\|FoldChange\|	基因起始位点	基因终止位点	注释
CmoCh01G000150	1.192 986 569	51 481	52 456	生长素相关蛋白
CmoCh02G009000	-2.729 158 581	5 550 636	5 554 600	生长素应答因子22
CmoCh02G015780	-1.085 102 287	9 109 056	9 112 669	生长素渠化
CmoCh04G004950	-2.026 235 227	2 470 212	2 472 488	生长素响应GH3样蛋白1
CmoCh04G006880	1.535 353 461	3 408 834	3 412 302	生长素诱导蛋白
CmoCh06G010560	-2.044 779 387	8 210 781	8 214 108	生长素响应GH3样蛋白5
CmoCh07G000800	-1.672 989 912	500 794	505 922	生长素应答因子
CmoCh07G002250	-2.181 053 727	1 136 983	1 140 904	生长素抑制蛋白
CmoCh07G007480	3.270 328 289	3 388 548	3 390 222	生长素应答因子36
CmoCh08G001220	-1.678 882 862	629 552	633 268	生长素反应蛋白IAA26
CmoCh08G007660	3.895 043 450	4 840 255	4 840 875	生长素结合蛋白
CmoCh08G011010	-1.027 143 495	7 056 498	7 063 138	生长素渠化
CmoCh09G006170	1.054 262 364	3 084 770	3 091 533	生长素渠化
CmoCh10G006720	-1.210 110 125	3 087 573	3 089 725	生长素反应蛋白IAA27
CmoCh11G006340	-1.374 547 937	3 047 847	3 051 459	生长素反应蛋白IAA27
CmoCh14G013230	-1.248 495 558	11 047 643	11 049 773	生长素反应蛋白IAA26
CmoCh15G011730	-3.433 307 646	8 165 466	8 169 541	生长素渠化
CmoCh15G013610	-1.282 714 907	9 303 666	9 310 054	生长素应答因子9
CmoCh16G005850	-1.222 754 998	2 822 610	2 826 352	生长素应答因子9
CmoCh17G013690	-1.441 604 240	10 631 575	10 632 190	生长素反应蛋白
CmoCh19G000230	1.584 356 252	139 153	141 383	生长素渠化
CmoCh19G007170	2.359 833 611	7 501 456	7 501 755	生长素反应蛋白SAUR50
CmoCh19G007210	1.222 892 554	7 514 737	7 515 027	生长素诱导蛋白
CmoCh20G005090	-1.540 231 132	2 433 984	2 440 115	生长素应答因子4
CmoCh20G008040	2.795 275 878	3 960 038	3 962 188	生长素反应蛋白SAUR24

基因 ID	log₂\|FoldChange\|	基因起始位点	基因终止位点	注释
CmoCh01G000150	1.192 986 569	51 481	52 456	生长素相关蛋白
CmoCh02G009000	-2.729 158 581	5 550 636	5 554 600	生长素应答因子22
CmoCh02G015780	-1.085 102 287	9 109 056	9 112 669	生长素渠化
CmoCh04G004950	-2.026 235 227	2 470 212	2 472 488	生长素响应GH3样蛋白1
CmoCh04G006880	1.535 353 461	3 408 834	3 412 302	生长素诱导蛋白
CmoCh06G010560	-2.044 779 387	8 210 781	8 214 108	生长素响应GH3样蛋白5
CmoCh07G000800	-1.672 989 912	500 794	505 922	生长素应答因子
CmoCh07G002250	-2.181 053 727	1 136 983	1 140 904	生长素抑制蛋白
CmoCh07G007480	3.270 328 289	3 388 548	3 390 222	生长素应答因子36
CmoCh08G001220	-1.678 882 862	629 552	633 268	生长素反应蛋白IAA26
CmoCh08G007660	3.895 043 450	4 840 255	4 840 875	生长素结合蛋白
CmoCh08G011010	-1.027 143 495	7 056 498	7 063 138	生长素渠化
CmoCh09G006170	1.054 262 364	3 084 770	3 091 533	生长素渠化
CmoCh10G006720	-1.210 110 125	3 087 573	3 089 725	生长素反应蛋白IAA27
CmoCh11G006340	-1.374 547 937	3 047 847	3 051 459	生长素反应蛋白IAA27
CmoCh14G013230	-1.248 495 558	11 047 643	11 049 773	生长素反应蛋白IAA26
CmoCh15G011730	-3.433 307 646	8 165 466	8 169 541	生长素渠化
CmoCh15G013610	-1.282 714 907	9 303 666	9 310 054	生长素应答因子9
CmoCh16G005850	-1.222 754 998	2 822 610	2 826 352	生长素应答因子9
CmoCh17G013690	-1.441 604 240	10 631 575	10 632 190	生长素反应蛋白
CmoCh19G000230	1.584 356 252	139 153	141 383	生长素渠化
CmoCh19G007170	2.359 833 611	7 501 456	7 501 755	生长素反应蛋白SAUR50
CmoCh19G007210	1.222 892 554	7 514 737	7 515 027	生长素诱导蛋白
CmoCh20G005090	-1.540 231 132	2 433 984	2 440 115	生长素应答因子4
CmoCh20G008040	2.795 275 878	3 960 038	3 962 188	生长素反应蛋白SAUR24

基因ID	甲基化类型	甲基化区域	基因ID	甲基化类型	甲基化区域
CmoCh01G010370	CHH	内含子	CmoCh13G008530	CHH	启动子
CmoCh02G003140	CHH	内含子	CmoCh14G006160	CHH	内含子
CmoCh03G012750	CHH	启动子	CmoCh14G006150	CHH	内含子
CmoCh04G007210	CHH	启动子	CmoCh14G013230	CHH	内含子
CmoCh04G029470	CHH	启动子	CmoCh14G015800	CHH	启动子
CmoCh04G030360	CHH	启动子	CmoCh14G018090	CHH	启动子
CmoCh05G007670	CHH, CG	内含子, 外显子	CmoCh15G001380	CHH	内含子
CmoCh05G008000	CHH	启动子	CmoCh15G009810	CHH	启动子
CmoCh07G002150	CHH	启动子	CmoCh17G001350	CHH	启动子
CmoCh07G009450	CHH	启动子	CmoCh17G011930	CG, CHH	启动子
CmoCh07G010990	CHH	启动子	CmoCh18G004680	CHH	内含子
CmoCh09G001100	CHH	启动子	CmoCh18G010050	CHH	内含子
CmoCh09G004620	CHH	内含子	CmoCh19G001290	CHH	启动子
CmoCh10G005700	CHH	启动子	CmoCh19G007160	CHH	启动子
CmoCh11G003520	CG	外显子	CmoCh19G007170	CHH	启动子
CmoCh11G007400	CG	启动子	CmoCh19G007180	CHH	启动子
CmoCh11G015800	CHH	启动子	CmoCh19G007290	CG	启动子
CmoCh11G017120	CHH	启动子	CmoCh19G007400	CHH	内含子, 启动子
CmoCh11G017130	CHH	启动子	CmoCh20G005420	CHH	启动子
CmoCh11G017140	CHH	启动子	CmoCh20G007770	CHH	启动子
CmoCh11G017180	CHH	启动子	CmoCh20G007790	CHH	启动子
CmoCh11G017330	CG, CHG	内含子, 外显子	CmoCh20G007780	CHH	内含子
CmoCh11G017660	CHH	启动子	CmoCh20G008020	CG	启动子
CmoCh12G001120	CHH	内含子, 启动子	CmoCh20G008010	CG	启动子
CmoCh12G003660	CHH	启动子	CmoCh20G008070	CHH	启动子
CmoCh12G011430	CHH	启动子	CmoCh20G008080	CHH	启动子
CmoCh12G013660	CHH	启动子

基因ID	甲基化类型	甲基化区域	基因ID	甲基化类型	甲基化区域
CmoCh01G010370	CHH	内含子	CmoCh13G008530	CHH	启动子
CmoCh02G003140	CHH	内含子	CmoCh14G006160	CHH	内含子
CmoCh03G012750	CHH	启动子	CmoCh14G006150	CHH	内含子
CmoCh04G007210	CHH	启动子	CmoCh14G013230	CHH	内含子
CmoCh04G029470	CHH	启动子	CmoCh14G015800	CHH	启动子
CmoCh04G030360	CHH	启动子	CmoCh14G018090	CHH	启动子
CmoCh05G007670	CHH, CG	内含子, 外显子	CmoCh15G001380	CHH	内含子
CmoCh05G008000	CHH	启动子	CmoCh15G009810	CHH	启动子
CmoCh07G002150	CHH	启动子	CmoCh17G001350	CHH	启动子
CmoCh07G009450	CHH	启动子	CmoCh17G011930	CG, CHH	启动子
CmoCh07G010990	CHH	启动子	CmoCh18G004680	CHH	内含子
CmoCh09G001100	CHH	启动子	CmoCh18G010050	CHH	内含子
CmoCh09G004620	CHH	内含子	CmoCh19G001290	CHH	启动子
CmoCh10G005700	CHH	启动子	CmoCh19G007160	CHH	启动子
CmoCh11G003520	CG	外显子	CmoCh19G007170	CHH	启动子
CmoCh11G007400	CG	启动子	CmoCh19G007180	CHH	启动子
CmoCh11G015800	CHH	启动子	CmoCh19G007290	CG	启动子
CmoCh11G017120	CHH	启动子	CmoCh19G007400	CHH	内含子, 启动子
CmoCh11G017130	CHH	启动子	CmoCh20G005420	CHH	启动子
CmoCh11G017140	CHH	启动子	CmoCh20G007770	CHH	启动子
CmoCh11G017180	CHH	启动子	CmoCh20G007790	CHH	启动子
CmoCh11G017330	CG, CHG	内含子, 外显子	CmoCh20G007780	CHH	内含子
CmoCh11G017660	CHH	启动子	CmoCh20G008020	CG	启动子
CmoCh12G001120	CHH	内含子, 启动子	CmoCh20G008010	CG	启动子
CmoCh12G003660	CHH	启动子	CmoCh20G008070	CHH	启动子
CmoCh12G011430	CHH	启动子	CmoCh20G008080	CHH	启动子
CmoCh12G013660	CHH	启动子

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生长素信号途径参与南瓜白粉菌胁迫的响应研究

Study on the Involvement of Auxin Signaling Pathway in Response to Powdery Mildew Stress in Pumpkin

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