关键词: 铅, 邻苯二甲酸二丁酯, 联合暴露, 斑马鱼胚胎, 基因表达

Abstract: The aim of the research was to study the effect of individual and combined exposure to lead (Pb）and dinbutyl phthalate(DBP) on the neurological and apoptosis-related gene expressions, and the effect on the nervous system of zebra fish embryos. According to the 3×3 factorial design, 720 zebra fish embryos were randomly divided into 9 groups, Pb (0, 0.01 mg/L,1 mg/L) and DBP (0, 0.005 mg/L,0.5 mg/L) were administered individually and mixtures to zebra fish embryos for 120 h, hatchability and mortality were record. Quantitative real-time PCR was used to assess neurological gene (NR1A, NR2A, NR2D) and apoptosis-related gene (bcl-2, c-fos) expressions. Exposure to individual and combined of Pb and DBP could cause decrease in hatchability and increase in mortality. There was an interaction between Pb and DBP on hatchability and mortality（P＜0.05). Co-exposure to Pb and DBP up-regulated  NR1A, NR2A, NR2D, c-fos gene and down-regulated bcl-2 gene (P<0.05). Exposure to Pb and DBP could impaire the development of zebra fish embryos and cause a certain neurotoxicity. The joint toxicity of them was complicated. It has interaction effect on the hatching rate and mortality and no interaction on neurological gene and apoptosis related genes of zebra fish embryos. The mechanism needs to be further studied.

Key words: lead, dibutyl phthalate, combined exposure, embryo of Danio rerio, gene expression