关键词: 不孕不育症, FSH-CTP, 分离纯化, 雌性大鼠卵巢增重法

Abstract: To obtain high purity recombinant human long-acting follicle stimulating hormone FSH-CTP, the study collected the CHO cells culture supernatant contained FSH-CTP, then it was puried by dye affinity chromatography, DEAE anion exchange chromatography and hydrophobic interaction chromatography. We detected the puried samples by molecular weight, isoelectric point, biological activity in vitro (Western Blot) and biological activity in vivo (ovarian weight gain in female rats law). The results showed that: The purity of FSH-CTP protein could reach 98% after three stept purification,  the apparent molecular weight was about 47 kDa, MALDI-TOF-MS result was 35.2 kDa. The results of isoelectric point was 4.0~2.8. And we provided the FSH-CTP activity was higher than recombinant human follicle stimulating hormone significantly by Western Blot analysis. The purification and activity of FSH-CTP was high. The results could lay the foundation for further study of the drug.

Key words: infertility, FSH-CTP, isolation and purification, ovarian weight increase in female rat